NMM Human
Greater than 95.0% as determined by SDS-PAGE.
Description
The NMM is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Non-Muscle Myosin-II (NMII) is a crucial molecular motor involved in various cellular processes. It is a part of the myosin superfamily, which consists of actin-based molecular motors that convert chemical energy into mechanical work. NMII is particularly significant in non-muscle cells, where it plays a vital role in cell adhesion, migration, and division.
NMII is a hexamer composed of two heavy chains, two essential light chains (ELCs), and two regulatory light chains (RLCs) . The regulatory light chains are approximately 20 kDa in size and are critical for the regulation of NMII activity. The heavy chains form the backbone of the molecule, while the light chains modulate its function.
The motor activity of NMII involves binding to F-actin, hydrolysis of ATP, and a resulting power stroke. This process is known as the myosin mechanochemical cycle or the cross-bridge cycle . The regulatory light chains play a pivotal role in this cycle by modulating the interaction between the myosin heads and actin filaments. Phosphorylation of the RLCs is a key regulatory mechanism that controls NMII activity .
NMII is involved in a plethora of cellular processes, including:
- Cell Migration: NMII generates contractile forces that drive cell movement.
- Cytokinesis: During cell division, NMII helps in the formation of the contractile ring, which is essential for the separation of daughter cells.
- Epithelial Barrier Function: NMII contributes to the maintenance of tight junctions and the integrity of epithelial layers.
- Tissue Morphogenesis: NMII is involved in shaping tissues during development by regulating cell shape and movement .
The human recombinant NMII regulatory light chain is a biotechnologically produced version of the natural protein. It is used in various research applications to study the function and regulation of NMII. Recombinant proteins are produced using genetic engineering techniques, where the gene encoding the protein is inserted into an expression system, such as bacteria or yeast, to produce large quantities of the protein.
Research on NMII and its regulatory light chains has provided significant insights into the molecular mechanisms underlying various cellular processes. Advanced imaging technologies and biophysical approaches have revealed new aspects of NMII assembly and function . The human recombinant NMII regulatory light chain is a valuable tool in these studies, allowing researchers to dissect the specific roles of RLCs in NMII regulation.
