NME2 Human, Active
Nucleoside diphosphate kinase B, NDPK-B, NDPKB, NM23-H2, NM23B.
Greater than 90.0% as determined by SDS-PAGE.
Description
NME2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (1-152 a.a.) and having a molecular mass of 17.2kDa.
Product Specs
NME2, also known as Non-Metastatic Cells 2, is a protein that functions as a nucleoside diphosphate kinase. It has a heterodimeric structure consisting of two polypeptide chains, A and B, each composed of 152 amino acids. The beta subunit of erythrocyte NDP kinase shares an identical structure with NME2. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NME2 appears to play a role in regulating signal transduction pathways.
Recombinant human NME2, expressed in E. coli, is a single, non-glycosylated polypeptide chain containing 152 amino acids (amino acids 1-152). It has a molecular mass of 17.2 kDa.
The product appears as a sterile, colorless solution.
The NME2 solution is provided at a concentration of 1 mg/ml and contains 10% glycerol, 20 mM Tris-HCl buffer (pH 8.0), and 1 mM DTT.
For short-term storage (2-4 weeks), the product should be stored at 4°C. For longer storage, it is recommended to freeze the product at -20°C. To ensure stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Multiple freeze-thaw cycles should be avoided.
The purity of the product is determined to be greater than 90.0% using SDS-PAGE analysis.
The specific activity of the enzyme is greater than 1,800 units/mg. Specific activity is defined as the amount of enzyme required to convert 1.0 µmol of both ATP and TDP to ADP and TTP per minute at a pH of 7.5 and a temperature of 25°C in a coupled system with PK/LDH.
Nucleoside diphosphate kinase B, NDPK-B, NDPKB, NM23-H2, NM23B.
MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE
Product Science Overview
NME2 is a heterodimeric protein composed of two polypeptide chains, A and B, each containing 152 amino acids. The protein has a molecular mass of approximately 17.2 kDa . It acts as a nucleoside diphosphate kinase, transferring the gamma phosphate from ATP to the beta phosphate of nucleoside diphosphates (NDPs) through a ping-pong mechanism involving a phosphorylated active-site intermediate .
The specific activity of NME2 is greater than 1,800 units per milligram, defined as the amount of enzyme that converts 1.0 micromole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25°C in a coupled system with pyruvate kinase (PK) and lactate dehydrogenase (LDH) . This activity is crucial for maintaining the balance of nucleoside triphosphates within the cell.
The human recombinant NME2 is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain . For laboratory research purposes, it is provided as a sterile, filtered, colorless solution containing 10% glycerol, 20mM Tris-HCl buffer (pH 8.0), and 1mM DTT . The protein is stable when stored at 4°C for short-term use (2-4 weeks) and at -20°C for long-term storage, with the addition of a carrier protein to prevent multiple freeze-thaw cycles .
