NMB Human

The genes encoding Neuromedin B and its receptor (NMBR) have been cloned and analyzed in various species. Studies have shown that the amino acid sequences and gene structures of NMB and NMBR are highly conserved across different species . This conservation suggests that Neuromedin B has essential physiological roles that have been maintained throughout evolution.

Neuromedin B performs a variety of physiological functions through its interaction with the NMBR. These functions include:

• Smooth Muscle Contraction: NMB stimulates smooth muscle contraction in a manner similar to bombesin .
• Reproductive Functions: NMB and NMBR are expressed in the reproductive axis, with varying expression levels during different phases of the estrous cycle in females and postnatal development stages in males .
• Central Nervous System: NMB is primarily expressed in the CNS, where it may play a role in regulating various neurological functions .

Recombinant human Neuromedin B is produced using recombinant DNA technology. This involves inserting the gene encoding NMB into a suitable expression system, such as E. coli, to produce the peptide in large quantities. The recombinant protein is often tagged with a His-tag to facilitate purification and detection .

The recombinant human Neuromedin B protein is used in various research applications to study its physiological functions and potential therapeutic uses. It is essential for understanding the role of NMB in health and disease and for developing potential treatments targeting the NMB/NMBR system.

Recombinant human Neuromedin B is typically stored in a buffer solution at -20°C for long-term storage. It is essential to avoid freeze-thaw cycles to maintain the protein’s stability and functionality .