NKp30 Antibody

Natural killer (NK) cells are a crucial component of the innate immune system, responsible for the early defense against virus-infected and malignantly transformed cells. Among the receptors that facilitate NK cell function, the natural killer cell p30-related protein (NKp30) plays a significant role. NKp30, also known as CD337 or NCR3, is a type I transmembrane protein that is part of the natural cytotoxicity receptors (NCRs) family .

NKp30 is characterized by a single extracellular V-like immunoglobulin domain . It interacts with CD3-zeta (CD247), a T-cell receptor, to mediate the cytotoxic activity of NK cells . This interaction is crucial for the lysis of tumor cells and the elimination of virus-infected cells. NKp30 is involved in the recognition and destruction of these abnormal cells, making it a vital player in immune surveillance .

A single nucleotide polymorphism in the 5’ untranslated region of the NKp30 gene has been linked to mild malaria susceptibility . This highlights the genetic diversity and potential implications of NKp30 in various immune responses and disease susceptibilities.

The mouse anti-human NKp30 antibody is derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NKp30 amino acids 19-138 purified from E. coli . The resulting monoclonal antibody is of the IgG1 subclass and is purified from mouse ascitic fluids by protein-G affinity chromatography .

The NKp30 antibody has been tested by ELISA and Western blot analysis to ensure specificity and reactivity . It is used in various research applications to study NK cell function, tumor immunology, and immune responses. The recommended dilution range for Western blot analysis is 1:500 to 2,000, with a starting dilution of 1:1,000 .

For optimal stability, the NKp30 antibody should be stored at 4°C for up to one month or at -20°C for longer periods . It is important to prevent freeze-thaw cycles to maintain the antibody’s integrity and functionality .