NKIRAS1 Human
NFKB Inhibitor Interacting Ras-Like 1 Human Recombinant
Cat. No.
BT18464
Source
Escherichia Coli.
Synonyms
NF-kappa-B inhibitor-interacting Ras-like protein 1, I-kappa-B-interacting Ras-like protein 1, Kappa B-Ras protein 1, KappaB-Ras1, NKIRAS1, KBRAS1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
NKIRAS1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 212 amino acids (1-192 a.a) and having a molecular mass of 23.8kDa (Molecular weight on SDS-PAGE will appear higher).
NKIRAS1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
NKIRAS1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
NKIRAS1, or NF-kappa-B inhibitor-interacting Ras-like protein 1, belongs to the small GTPase family. This unique Ras-like protein acts as a powerful regulator of NF-kappa-B activity. It achieves this by preventing the breakdown of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. NKIRAS1 operates by blocking NFKBIB phosphorylation and mediating the cytoplasmic retention of the p65/RELA NF-kappa-B subunit. Both GTP- and GDP-bound forms of NKIRAS1 are capable of blocking NFKBIB phosphorylation.
Description
Recombinant human NKIRAS1, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 212 amino acids (amino acids 1-192) and has a molecular weight of 23.8kDa. Note that the molecular weight observed on SDS-PAGE will be higher. This NKIRAS1 protein is fused to a 20 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The NKIRAS1 protein solution (0.25mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 20% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), keep the vial refrigerated at 4°C. For longer storage, freeze the protein at -20°C. For optimal long-term storage, we recommend adding a carrier protein (0.1% HSA or BSA). Avoid repeated freezing and thawing of the protein.
Purity
The purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Synonyms
NF-kappa-B inhibitor-interacting Ras-like protein 1, I-kappa-B-interacting Ras-like protein 1, Kappa B-Ras protein 1, KappaB-Ras1, NKIRAS1, KBRAS1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGKGCKVVVC GLLSVGKTAI LEQLLYGNHT IGMEDCETME DVYMASVETD RGVKEQLHLY DTRGLQEGVE LPKHYFSFAD GFVLVYSVNN LESFQRVELL KKEIDKFKDK KEVAIVVLGN KIDLSEQRQV DAEVAQQWAK SEKVRLWEVT VTDRKTLIEP FTLLASKLSQ PQSKSSFPLP GRKNKGNSNS EN.
Product Science Overview
Gene and Protein Structure
The NKIRAS1 gene is located on chromosome 3 and is a protein-coding gene . The protein itself is an atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity . It achieves this by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, which explains why NFKBIB is more resistant to degradation .
Biological Function
NKIRAS1 plays a crucial role in the I-kappaB kinase/NF-kappaB signaling pathway . This pathway is essential for regulating immune responses, inflammation, and cell survival. NKIRAS1 is predicted to enable GTPase activating protein binding activity and is involved in several processes, including Ral protein signal transduction, lung alveolus development, and surfactant homeostasis .
Mechanism of Action
Clinical Significance
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