NIT2 Human
Description
NIT2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Nitrilase Family Member 2 (NIT2) is a protein-coding gene that belongs to the nitrilase family. This family of enzymes is known for its role in catalyzing the hydrolysis of nitriles to carboxylic acids and ammonia, a process that is significant in various biological and industrial applications . NIT2, in particular, has been identified as having omega-amidase activity, which is crucial for the metabolism of certain amino acids and the detoxification of harmful intermediates .
The NIT2 gene is located on chromosome 3 in humans and is expressed in various tissues throughout the body . The protein encoded by this gene is involved in several metabolic pathways, including the asparagine and glutamine metabolic processes . The enzyme’s structure allows it to act on carbon-nitrogen bonds, excluding peptide bonds, which is a characteristic feature of the nitrilase family .
NIT2’s primary function is to act as an omega-amidase. This enzyme plays a vital role in converting potentially toxic intermediates, such as 2-oxoglutaramate and 2-oxosuccinamate, into biologically useful compounds like 2-oxoglutarate and oxaloacetate . These conversions are essential for maintaining cellular homeostasis and preventing the accumulation of harmful substances within the cell .
The recombinant form of NIT2, produced through genetic engineering techniques, has significant applications in both research and industry. In research, it is used to study the metabolic pathways involving asparagine and glutamine, providing insights into various physiological and pathological processes . In industry, NIT2’s ability to catalyze the hydrolysis of nitriles makes it a valuable tool in the production of carboxylic acids, which are important intermediates in the synthesis of pharmaceuticals, agrochemicals, and other fine chemicals .
Mutations or dysregulation of the NIT2 gene have been associated with certain diseases, including acute allergic sanguinous otitis media and developmental and epileptic encephalopathy . Understanding the function and regulation of NIT2 can therefore contribute to the development of therapeutic strategies for these conditions.
