NIP7 Human

Nuclear Import 7 Homolog, also known as NIP7, is a protein that plays a crucial role in the biogenesis of the 60S ribosomal subunit. This protein is essential for the proper assembly and function of ribosomes, which are the cellular machinery responsible for protein synthesis. The recombinant form of this protein, produced in Escherichia coli, is often used in research to study its function and interactions.

The recombinant Human Nuclear Import 7 Homolog is a single, non-glycosylated polypeptide chain consisting of 188 amino acids. It has a molecular mass of approximately 21.5 kDa . The protein solution typically contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, and 0.1M NaCl . For long-term storage, it is recommended to add a carrier protein such as 0.1% Human Serum Albumin (HSA) or Bovine Serum Albumin (BSA) to prevent degradation .

NIP7 is involved in the nuclear import of ribosomal proteins and other macromolecules. It interacts with import receptors and nucleoporins to facilitate the transport of these molecules through the nuclear pore complex (NPC). This process is vital for maintaining cellular function and ensuring the proper assembly of ribosomes .

The nuclear import of proteins like NIP7 involves several steps:

1. Recognition and Binding: NIP7 is recognized by import receptors such as importin 7. These receptors bind to the nuclear localization signals (NLS) present on the NIP7 protein .
2. Transport through NPC: The import receptor-NIP7 complex is transported through the NPC. Nucleoporins, such as Nup358, play a crucial role in this process by interacting with the import receptor and facilitating the translocation of the complex into the nucleus .
3. Release and Recycling: Once inside the nucleus, the NIP7 protein is released from the import receptor, which is then recycled back to the cytoplasm to transport more proteins .

Recombinant NIP7 is widely used in research to study its role in ribosome biogenesis and nuclear import. It is also used to investigate the interactions between import receptors and nucleoporins, as well as the mechanisms underlying nuclear transport. Understanding these processes can provide insights into various cellular functions and diseases related to ribosome assembly and protein synthesis.

For optimal stability, the recombinant NIP7 protein should be stored at 4°C if it will be used within 2-4 weeks. For longer storage periods, it should be frozen at -20°C. It is important to avoid multiple freeze-thaw cycles to prevent protein degradation .