NHLH2 Human

Nescient Helix Loop Helix 2 Human Recombinant
Cat. No.
BT17119
Source
Escherichia Coli.
Synonyms
nescient helix loop helix 2, bHLHa34, HEN2, NSCL2, Class A basic helix-loop-helix protein 34, NHLH2, KIAA0490.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NHLH2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids (1-135 a.a.) and having a molecular mass of 17.4kDa.
NHLH2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Nescient Helix Loop Helix 2 (NHLH2) is part of the basic helix-loop-helix (bHLH) family of transcription factors. It is found in developing and mature neuroendocrine tissues, such as the pituitary and hypothalamus. NHLH2 functions as a DNA-binding protein and may play a role in controlling cell fate, particularly in the developing nervous system.
Description
NHLH2 Human Recombinant, manufactured in E.Coli, is a single, non-glycosylated polypeptide chain comprising 158 amino acids (1-135 a.a.) with a molecular weight of 17.4kDa. This protein is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
The product is a clear solution that has been sterilized through filtration.
Formulation
The NHLH2 protein solution (0.25mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 7.5), 0.2M NaCl, 50% glycerol, and 5mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance long-term stability, consider adding a carrier protein like HSA or BSA (0.1%). It is important to minimize repeated freezing and thawing of the product.
Purity
Analysis by SDS-PAGE indicates a purity level exceeding 90.0%.
Synonyms
nescient helix loop helix 2, bHLHa34, HEN2, NSCL2, Class A basic helix-loop-helix protein 34, NHLH2, KIAA0490.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMMLSPDQ AADSDHPSSA HSDPESLGGT DTKVLGSVSD LEPVEEAEGD GKGGSRAALY PHPQQLSREE KRRRRRATAK YRSAHATRER IRVEAFNLAF AELRKLLPTL PPDKKLSKIE ILRLAICYIS YLNHVLDV.

Product Science Overview

Introduction

Nescient Helix Loop Helix 2 (NHLH2), also known as HEN2, is a protein-coding gene that plays a crucial role in various biological processes. This gene encodes a transcription factor that is involved in the regulation of gene expression, particularly in the hypothalamus, which is a critical region for maintaining energy homeostasis and reproductive functions.

Gene and Protein Structure

NHLH2 belongs to the basic helix-loop-helix (bHLH) family of transcription factors. These proteins are characterized by a conserved domain that facilitates DNA binding and dimerization. The NHLH2 protein binds to specific DNA sequences known as E-box motifs, which are essential for the transcriptional regulation of target genes .

Biological Functions

NHLH2 is predominantly expressed in the hypothalamus and is involved in several key physiological processes:

  1. Energy Homeostasis: NHLH2 regulates the expression of prohormone convertase 1/3 (PC1/3), an enzyme that processes proopiomelanocortin (POMC) into active peptides. These peptides play a significant role in controlling appetite and energy expenditure .
  2. Reproductive Functions: NHLH2 is involved in the development and function of the hypothalamic-pituitary-gonadal axis, which is crucial for reproductive longevity and mating behavior .
  3. Neuronal Development: NHLH2 is required for the migration and survival of neurons in the anterior extramural migration stream, which forms the precerebellar nuclei .
Mechanisms of Action

NHLH2 functions as a transcriptional activator by binding to E-box motifs in the promoter regions of target genes. It interacts with other transcription factors, such as Signal Transducer and Activator of Transcription 3 (STAT3), to regulate gene expression. For instance, NHLH2 and STAT3 form a heterodimer that binds to the promoter of the PC1/3 gene, enhancing its transcription in response to leptin signaling .

Clinical Significance

Mutations or dysregulation of NHLH2 have been associated with various disorders, including:

  1. Hypogonadotropic Hypogonadism: This condition is characterized by reduced secretion of gonadotropins, leading to impaired sexual development and reproductive function .
  2. Energy Balance Disorders: Given its role in energy homeostasis, alterations in NHLH2 expression can contribute to obesity and related metabolic disorders .
Research and Therapeutic Potential

Research on NHLH2 continues to uncover its diverse roles in physiology and disease. Understanding the mechanisms by which NHLH2 regulates gene expression and its interactions with other signaling pathways could lead to novel therapeutic strategies for treating metabolic and reproductive disorders.

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