NFATC2 Human
Nuclear Factor Of Activated T Cells 2, Nuclear Factor Of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2, Nuclear Factor Of Activated T-Cells 2, NFAT Pre-Existing Subunit, NF-ATc2, NFAT1, NFATP, Nuclear Factor Of Activated T-Cells, Preexisting Component , Nuclear Factor Of Activated T-Cells, Cytoplasmic 2, NFAT Transcription Complex, Preexisting Component, Preexisting Nuclear Factor Of Activated T-Cells 2, T Cell Transcription Factor NFAT1, T-Cell Transcription Factor NFAT1, NF-ATp, NFATc2.
Greater than 90.0% as determined by SDS-PAGE.
Description
NFATC2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 290 amino acids (396-678a.a.) and having a molecular mass of 33.1kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). NFATC2 is expressed with a 7 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
NFATC2, alternatively known as nuclear factor of activated T-cells 2, is a member of the nuclear factor of activated T cells family. This protein plays a crucial role in T helper cell differentiation, activation, and effector function. While knockout of a single NFAT isoform in T cells results in relatively minor effects, T cells lacking both NFATC1 and NFATC2 completely lose the ability to produce T helper cell effector cytokines, such as interleukins IL-4 and IL-2. NFATC2 is typically found in a highly phosphorylated state within the cytoplasm. Upon T cell receptor stimulation, calcium-activated calcineurin dephosphorylates NFATC2, leading to a conformational change that exposes nuclear localization sequences.
Produced in Sf9 Baculovirus cells, NFATC2 is a single, glycosylated polypeptide chain consisting of 290 amino acids (396-678a.a.). It has a molecular weight of 33.1 kDa. It's important to note that on SDS-PAGE, the apparent molecular size will be approximately 28-40 kDa. This NFATC2 protein is expressed with a 7 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques.
The NFATC2 protein solution is provided at a concentration of 0.25 mg/ml and is formulated in a buffer containing Phosphate Buffered Saline (pH 7.4), 40% glycerol, and 1mM DTT.
The purity of NFATC2 is determined to be greater than 90.0% as assessed by SDS-PAGE analysis.
Nuclear Factor Of Activated T Cells 2, Nuclear Factor Of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2, Nuclear Factor Of Activated T-Cells 2, NFAT Pre-Existing Subunit, NF-ATc2, NFAT1, NFATP, Nuclear Factor Of Activated T-Cells, Preexisting Component , Nuclear Factor Of Activated T-Cells, Cytoplasmic 2, NFAT Transcription Complex, Preexisting Component, Preexisting Nuclear Factor Of Activated T-Cells 2, T Cell Transcription Factor NFAT1, T-Cell Transcription Factor NFAT1, NF-ATp, NFATc2.
MPLEWPLSSQ SGSYELRIEV QPKPHHRAHY ETEGSRGAVK APTGGHPVVQ LHGYMENKPL GLQIFIGTAD ERILKPHAFY QVHRITGKTV TTTSYEKIVG NTKVLEIPLE PKNNMRATID CAGILKLRNA DIELRKGETD IGRKNTRVRL VFRVHIPESS GRIVSLQTAS NPIECSQRSA HELPMVERQD TDSCLVYGGQ QMILTGQNFT SESKVVFTEK TTDGQQIWEM EATVDKDKSQ PNMLFVEIPE YRNKHIRTPV KVNFYVINGK RKRSQPQHFT YHPVHHHHHH
Product Science Overview
Nuclear Factor of Activated T Cells 2 (NFATC2) is a member of the NFAT family of transcription factors, which play a crucial role in the regulation of gene expression during the immune response. NFATC2 is a DNA-binding protein characterized by a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is predominantly found in the cytosol and translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes part of the NFAT transcription complex .
NFATC2 is integral to the inducible expression of cytokine genes in T-cells, particularly in the induction of interleukins such as IL-2, IL-3, IL-4, TNF-alpha, and GM-CSF . This transcription factor is essential for the immune response, as it activates gene transcription necessary for T-cell activation and differentiation. Additionally, NFATC2 has been implicated in promoting invasive migration through the activation of GPC6 expression and the WNT5A signaling pathway .
Mutations or dysregulation of NFATC2 have been associated with various diseases, including joint contractures, osteochondromas, B-cell lymphoma, and soft tissue sarcoma . The translocation forming an in-frame fusion product between the EWSR1 gene and the NFATC2 gene has been described in bone tumors with a Ewing sarcoma-like clinical appearance . This translocation leads to the loss of controlling elements of the NFATC2 protein, resulting in constant activation of the protein.
The study of NFATC2 has expanded beyond its role in T-cells to include its involvement in other body systems. For instance, NFATC2 has been shown to interact with various proteins such as MEF2D, EP300, IRF4, and Protein kinase Mζ . These interactions highlight the diverse regulatory functions of NFATC2 in different cellular contexts.
