NDUFV3 Human
Description
NDUFV3 is fused to a 23 amino acid His-
tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
NDUFV3 is a 108 amino acid protein that is believed not to be involved in catalysis . Instead, it plays a structural role within the complex. Mitochondrial complex I functions by oxidizing NADH produced in the Krebs cycle, utilizing the two electrons to reduce ubiquinone to ubiquinol. This initiates the passage of electrons to successive complexes, ultimately leading to the reduction of oxygen to water .
Defects in any of the subunits of mitochondrial complex I, including NDUFV3, can lead to various myopathies and neuropathies . This makes it of considerable clinical interest. Although NDUFV3 itself is not directly involved in catalysis, its proper function is crucial for the stability and efficiency of the entire complex .
The recombinant form of NDUFV3 is typically expressed in Escherichia coli and purified using affinity chromatography techniques. The protein is often tagged with a His-ABP tag to facilitate purification . The purified protein is stored in a buffer containing 1M urea/PBS at pH 7.4 and is kept at -20°C to avoid freeze/thaw cycles .
NDUFV3 is used in various research applications, including studies on mitochondrial function and the electron transport chain. It serves as a control fragment in blocking experiments with corresponding antibodies . Researchers also use it to investigate the structural and functional aspects of mitochondrial complex I and its role in cellular respiration .
