NDUFA4 Human

NADH Dehydrogenase1 Alpha Subcomplex 4 Human Recombinant
Cat. No.
BT11982
Source
E.coli.
Synonyms
NDUFA4, Mitochondrial Complex Associated, NADH-Ubiquinone Oxidoreductase MLRQ Subunit, NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 4, 9kDa, Complex I 9kDa Subunit, Complex I-MLRQ, CI-MLRQ, NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 4 (9kD, MLRQ), NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Subunit 4, CI-9k, MLRQ, Cytochrome c oxidase subunit NDUFA4.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NDUFA4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 104 amino acids (1-81 a.a) and having a molecular mass of 11.8kDa.
NDUFA4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
NADH Dehydrogenase 1 Alpha Subcomplex 4 (NDUFA4) is a subunit of the mitochondrial respiratory chain complex I. This complex is responsible for transferring electrons from NADH to ubiquinone in the electron transport chain. NDUFA4 is a member of the complex I 9kDa subunit family and has NADH dehydrogenase and oxidoreductase activities.
Description
This product is a recombinant human NDUFA4 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain containing 104 amino acids (residues 1-81 of the native protein) with a molecular mass of 11.8 kDa. The protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The NDUFA4 protein is supplied at a concentration of 1 mg/ml in a solution containing 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, and 0.4 M Urea.
Stability
For short-term storage (2-4 weeks), store the protein at 4°C. For long-term storage, freeze the protein at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the NDUFA4 protein is greater than 80% as determined by SDS-PAGE analysis.
Synonyms
NDUFA4, Mitochondrial Complex Associated, NADH-Ubiquinone Oxidoreductase MLRQ Subunit, NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 4, 9kDa, Complex I 9kDa Subunit, Complex I-MLRQ, CI-MLRQ, NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 4 (9kD, MLRQ), NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Subunit 4, CI-9k, MLRQ, Cytochrome c oxidase subunit NDUFA4.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMLRQIIG QAKKHPSLIP LFVFIGTGAT GATLYLLRLA LFNPDVCWDR NNPEPWNKLG PNDQYKFYSV NVDYSKLKKE RPDF.

Product Science Overview

Introduction

NADH Dehydrogenase 1 Alpha Subcomplex 4 (NDUFA4) is a crucial component of the mitochondrial respiratory chain, specifically associated with Complex IV. This enzyme plays a vital role in cellular energy metabolism by participating in the electron transport chain, which is essential for the production of adenosine triphosphate (ATP) through oxidative phosphorylation (OXPHOS).

Gene and Protein Structure

NDUFA4 is encoded by the NDUFA4 gene located on human chromosome 7p21.3 . The protein is composed of 81 amino acids and is integral to the mitochondrial inner membrane. It functions as a part of the larger NADH dehydrogenase complex, facilitating the transfer of electrons from NADH to the respiratory chain .

Biological Function

NDUFA4 is essential for the proper functioning of the mitochondrial respiratory chain. It is involved in NADH dehydrogenase and oxidoreductase activities, transferring electrons from NADH to the respiratory chain. This process drives oxidative phosphorylation and is crucial for ATP production, respiratory electron transport, chemiosmotic coupling to ATP synthesis, and the generation of heat through the uncoupling of proteins .

Role in Disease

Alterations in the expression and function of NDUFA4 have been implicated in various diseases, including cancer. For instance, aberrant expression of NDUFA4 has been linked to gastrointestinal cancers such as colorectal, liver, and gastric cancers . The protein’s role in mitochondrial energy metabolism makes it a potential target for therapeutic interventions in these diseases.

Recombinant NDUFA4

Human recombinant NDUFA4 is produced using recombinant DNA technology, which involves inserting the human NDUFA4 gene into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of the protein for research and therapeutic purposes. Recombinant NDUFA4 is used in various studies to understand its function, structure, and role in disease, as well as to develop potential treatments targeting mitochondrial dysfunctions.

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