NDFIP1 Human

Nedd4 Family Interacting Protein 1 Human Recombinant
Cat. No.
BT28699
Source
Escherichia Coli.
Synonyms
N4WBP5, NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M, NEDD4 WW domain-binding protein 5, Putative MAPK-activating protein PM13, Putative NF-kappa-B-activating protein 164, Putative NFKB and MAPK-activating protein,NDFIP1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NDFIP1 Human Recombinant produced in E. coli is. a single polypeptide chain containing 139 amino acids (1-116) and having a molecular mass of 14.8kDa. NDFIP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
NDFIP1 (Nedd4 Family Interacting Protein 1) belongs to a small, evolutionarily conserved protein family characterized by three transmembrane domains. This protein is potentially ubiquitinated by the Nedd4 family of proteins. As a member of an integral Golgi membrane protein family, NDFIP1 also participates in modulating EGFR signaling through various pathways.
Description
Recombinant human NDFIP1, expressed in E. coli, is a single polypeptide chain consisting of 139 amino acids (residues 1-116) with a molecular weight of 14.8 kDa. An N-terminal 23-amino acid His-tag is fused to the protein, which is then purified using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The NDFIP1 solution is provided at a concentration of 1 mg/ml and is formulated in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 10% glycerol, and 0.1 M NaCl.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to store the solution frozen at -20°C. To ensure stability during long-term storage, adding a carrier protein like 0.1% HSA or BSA is advised. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of the protein is greater than 90% as assessed by SDS-PAGE analysis.
Synonyms
N4WBP5, NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M, NEDD4 WW domain-binding protein 5, Putative MAPK-activating protein PM13, Putative NF-kappa-B-activating protein 164, Putative NFKB and MAPK-activating protein,NDFIP1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMALALAA LAAVEPACGS RYQQLQNEEE SGEPEQAAGD APPPYSSISA ESAAYFDYKD ESGFPKPPSY NVATTLPSYD EAERTKAEAT IPLVPGRDED FVGRDDFDDA DQLRIGNDG.

Product Science Overview

Nedd4 Family and Ubiquitination

The Nedd4 family of E3 ubiquitin ligases plays a crucial role in the ubiquitination process, a post-translational modification that regulates various cellular functions, including protein degradation, signaling, and trafficking . The Nedd4 family includes several members, such as NEDD4-1, NEDD4-2, WWP1, WWP2, and ITCH, among others . These ligases recognize and modify proteins by adding ubiquitin molecules, which can signal for protein degradation via the proteasome or alter protein function and localization .

Role of NDFIP1

NDFIP1 is an integral Golgi membrane protein that interacts with Nedd4 family members . It is involved in various cellular processes, including the regulation of protein trafficking and degradation. NDFIP1 is recruited into exosomes, which are small vesicles involved in intercellular communication . This recruitment is essential for the regulation of protein sorting and secretion.

Clinical Relevance

NDFIP1 and its interactions with Nedd4 family members have significant implications in various diseases, particularly cancer. The Nedd4 family of E3 ligases is involved in the regulation of cell proliferation, migration, and invasion, making them critical players in tumorigenesis . Alterations in the expression or function of Nedd4 family members, including NDFIP1, can lead to dysregulation of these processes and contribute to cancer progression .

Research and Therapeutic Potential

Recent studies have highlighted the potential of targeting Nedd4 family members, including NDFIP1, for therapeutic purposes. Inhibitors or activators of Nedd4 ligases are being explored as potential treatments for cancer and other diseases . Understanding the specific interactions and functions of NDFIP1 in the context of the Nedd4 family can provide valuable insights into the development of novel therapeutic strategies.

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