NDE1 Human
Nuclear distribution protein nudE homolog 1, NudE, NDE1, NUDE, LIS4, NUDE1, HOM-TES-87.
Description
NDE1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 355 amino acids (1-335 a.a.) and having a molecular mass of 39.9kDa (Molecular weight on SDS-PAGE will appear higher).
NDE1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
NDE1, a cytoplasmic protein belonging to the nudE protein family, plays a crucial role in cell division and neuronal development. During mitosis, it undergoes phosphorylation, becoming essential for the proper assembly and function of the mitotic spindle. NDE1 regulates the Dynein-mediated transport of kinetochore proteins and contributes to centrosome duplication during interphase. This protein is known to interact with NDEL1, LIS1, and Dynein IC1/2 in the cytoplasm, forming a signaling pathway that governs neuron formation and is vital for cerebral cortex development.
This recombinant NDE1 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 355 amino acids (1-335 a.a.). It has a molecular mass of 39.9kDa (note: SDS-PAGE analysis may show a higher molecular weight due to protein migration characteristics). The protein includes a 20 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.
While NDE1 Human Recombinant remains stable for up to 1 week when stored at 4°C, it is recommended to store the protein below -18°C for long-term preservation. Repeated freezing and thawing should be avoided to maintain protein integrity.
Nuclear distribution protein nudE homolog 1, NudE, NDE1, NUDE, LIS4, NUDE1, HOM-TES-87.
MGSSHHHHHH SSGLVPRGSH MEDSGKTFSS EEEEANYWKD LAMTYKQRAE NTQEELREFQ EGSREYEAEL ETQLQQIETR NRDLLSENNR LRMELETIKE KFEVQHSEGY RQISALEDDL AQTKAIKDQL QKYIRELEQA NDDLERAKRA TIMSLEDFEQ RLNQAIERNA FLESELDEKE NLLESVQRLK DEARDLRQEL AVQQKQEKPR TPMPSSVEAE RTDTAVQATG SVPSTPIAHR GPSSSLNTPG SFRRGLDDST GGTPLTPAAR ISALNIVGDL LRKVGALESK LASCRNLVYD QSPNRTGGPA SGRSSKNRDG GERRPSSTSV PLGDKGLDTS CRWLSKSTTR SSSSC.
Product Science Overview
Nuclear distribution element 1 (NDE1), also known as NudE, is a protein encoded by the NDE1 gene in humans. This protein is a member of the nuclear distribution E (NudE) family and plays a crucial role in various cellular processes, particularly in the organization of microtubules, mitosis, and neuronal migration .
NDE1 is a coiled-coil-containing protein that localizes primarily at the centrosome, a key cellular structure involved in the organization of microtubules . During mitosis, NDE1 is partially associated with the mitotic spindle and concentrates at the plus ends of microtubules coincident with kinetochores in metaphase and anaphase . It also localizes to the cleavage furrow during cytokinesis .
NDE1 is essential for centrosome duplication and the formation and function of the mitotic spindle . It plays a critical role in the development of the cerebral cortex by regulating the orientation of the mitotic spindle during the division of cortical neuronal progenitors . This regulation is crucial for the production of neurons and the overall architecture of the brain.
NDE1 interacts with other centrosome components as part of a multiprotein complex that regulates dynein function . Dynein is a motor protein that moves along microtubules and is essential for various cellular processes, including the transport of organelles and vesicles, and the positioning of the mitotic spindle.
NDE1, along with its paralog NDE-like 1 (NDEL1), is critical for neurodevelopment and has been implicated in a range of psychiatric and neurodevelopmental disorders . Copy number variations (CNVs) at the 16p13.11 chromosomal locus, which contains the NDE1 gene, have been associated with intellectual disability, autism, attention deficit hyperactivity disorder, schizophrenia, and epilepsy . The interaction of NDE1 with Disrupted In Schizophrenia 1 (DISC1), a molecule strongly implicated in the risk of mental illness, further underscores its importance in mental health .
