NCL Human

Nucleolin Human Recombinant
Cat. No.
BT28028
Source
Sf9 Insect Cells.
Synonyms
Nucleolin, Protein C23, NCL, C23.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Nucleolin Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing the C-terminal section of the human nucleolin and missing the N-terminal histone-binding part of nucleolin, having a calculated molecular mass of 55,162 Dalton.
NCL is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Nucleolin (NCL) is a crucial eukaryotic nucleolar phosphoprotein that plays a vital role in ribosome synthesis and maturation. It is considered a key protein in the nucleolus of actively growing eukaryotic cells. NCL is found in association with intranucleolar chromatin and pre-ribosomal particles. It has been shown to induce chromatin decondensation by binding to histone H1. NCL is believed to be involved in pre-rRNA transcription and ribosome assembly. Additionally, it may contribute to the process of transcriptional elongation. NCL is primarily localized in the dense fibrillar regions of the nucleolus. The human NCL gene spans approximately 11kb and comprises 14 exons and 13 introns.
Description
Recombinant Human Nucleolin, produced in SF9 cells, is a glycosylated polypeptide chain containing the C-terminal section of human nucleolin, lacking the N-terminal histone-binding domain. It has a calculated molecular mass of 55,162 Daltons. This NCL protein is expressed with a -6x His tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
NCL is provided in a solution containing 20mM HEPES (pH 7.3), 600mM NaCl, 0.3mM Tris(2-carboxyethyl)phosphine (TCEP), and 25% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Repeated freezing and thawing should be avoided.
Purity
Purity is greater than 80.0%, as determined by SDS-PAGE analysis.
Synonyms
Nucleolin, Protein C23, NCL, C23.
Source
Sf9 Insect Cells.

Product Science Overview

Structure and Function

Nucleolin is composed of several structural domains that enable it to interact with different proteins and RNA sequences. It is an abundant protein in the nucleolus, accounting for up to 10% of the total nucleolar protein in some cells . The protein is approximately 100-110 kDa in size, although the predicted molecular mass based on its amino acid sequence is around 77 kDa .

The primary functions of nucleolin include:

  • Ribosome Biogenesis: Nucleolin is involved in the transcription of ribosomal RNA (rRNA), its modification, maturation, and assembly with ribosomal proteins to form pre-ribosomal particles .
  • Chromatin Structure Regulation: Nucleolin can induce chromatin decondensation by binding to histone H1, facilitating access to DNA for transcription .
  • RNA Metabolism: It binds to RNA oligonucleotides and is involved in various aspects of RNA processing and transport .
Biological Significance

Nucleolin is essential for the growth and proliferation of eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles, indicating its role in the early stages of ribosome assembly . Additionally, nucleolin has been implicated in the regulation of transcription by RNA polymerase I and II, angiogenesis, and cellular responses to various stimuli .

Clinical Relevance

Nucleolin has been studied for its potential role in various diseases. For instance, it acts as a low-affinity receptor for certain growth factors and can inhibit HIV infection by binding to the virus . Moreover, nucleolin at the cell surface serves as a receptor for the respiratory syncytial virus (RSV) fusion protein, highlighting its importance in viral infections .

Recombinant Nucleolin

Recombinant nucleolin is produced using genetic engineering techniques to express the human nucleolin protein in a host organism, such as bacteria or yeast. This allows for the large-scale production and purification of nucleolin for research and therapeutic purposes. Recombinant nucleolin retains the functional properties of the native protein and is used in various studies to understand its role in cellular processes and disease mechanisms.

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