NCK1 Human

NCK Adaptor Protein 1 Human Recombinant
Cat. No.
BT16837
Source
Escherichia Coli.
Synonyms
NCK, NCKalpha, NCK1, NCK adaptor protein 1, Cytoplasmic protein NCK1, SH2/SH3 adaptor protein NCK-alpha, MGC12668.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NCK1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 397 amino acids (1-377 a.a.) and having a molecular mass of 45 kDa. The NCK1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
NCK1, an adapter protein, interacts with tyrosine-phosphorylated growth factor receptors and their cellular substrates. It plays a crucial role in maintaining low levels of EIF2S1 phosphorylation by facilitating its dephosphorylation via PP1. Additionally, NCK1 participates in the DNA damage response pathway, ensuring efficient activation of downstream effectors like CHEK2. As a signaling and transforming protein, it possesses Src homology 2 and 3 (SH2 and SH3) domains. Located in the cytoplasm, NCK1 functions as an adaptor protein involved in signal transduction from receptor tyrosine kinases to downstream signal recipients, including RAS.
Description
Recombinant Human NCK1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 397 amino acids (1-377 a.a.). With a molecular mass of 45 kDa, it features a 20 amino acid His-Tag fused at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
The product is a clear, sterile-filtered solution.
Formulation
The provided solution of Human NCK1 contains 20mM Tris-HCl (pH 8), 1mM DTT, 1mM EDTA, 50mM NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, freezing at -20°C is recommended. To ensure optimal stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Repeated freezing and thawing cycles should be avoided.
Purity
The purity of the product, as determined by SDS-PAGE analysis, is greater than 90.0%.
Synonyms
NCK, NCKalpha, NCK1, NCK adaptor protein 1, Cytoplasmic protein NCK1, SH2/SH3 adaptor protein NCK-alpha, MGC12668.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGSYNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVIEKPENDPEWW KCRKINGMVG LVPKNYVTVM QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME ELVEHYKKAP IFTSEQGEKL YLVKHLS.

Product Science Overview

Introduction

NCK Adaptor Protein 1, also known as non-catalytic region of tyrosine kinase adaptor protein 1, is a crucial component in cellular signaling pathways. This protein is encoded by the NCK1 gene and is involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS .

Structure and Function

NCK1 is characterized by its modular structure, consisting of one Src homology 2 (SH2) domain and three Src homology 3 (SH3) domains . These domains facilitate the protein’s role as an adaptor, linking receptor and non-receptor tyrosine kinases to the actin cytoskeleton machinery. This connection is vital for the regulation of actin reorganization, which is essential for various cellular processes including cell migration, polarization, and immune responses .

Role in Cellular Processes

NCK1 plays a pivotal role in several cellular processes:

  • Actin Cytoskeleton Reorganization: NCK1 is involved in the reorganization of the actin cytoskeleton, which is crucial for cell movement and stability .
  • Signal Transduction: It acts as a mediator in signal transduction pathways, particularly those initiated by receptor tyrosine kinases .
  • Immune Response: In T lymphocytes, NCK1 is essential for the reorganization of the actin cytoskeleton during the formation of the immunological synapse, a critical step in the immune response .
Genetic and Functional Redundancy

The NCK family comprises two members: NCK1 and NCK2. These proteins share 68% identity at the amino acid level and are functionally redundant in many aspects . However, they also exhibit unique functions in certain cellular contexts. For instance, NCK2 has been shown to exclusively regulate actin polymerization in response to platelet-derived growth factor and epidermal growth factor in specific cell types .

Clinical Relevance

Mutations or dysregulation of NCK1 can be associated with various diseases. For example, NCK1 has been linked to Wiskott-Aldrich Syndrome and bone chondrosarcoma . Understanding the role of NCK1 in these conditions can provide insights into potential therapeutic targets.

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Source
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