NCF4 Human

Neutrophil Cytosolic Factor 4 Human Recombinant
Cat. No.
BT18102
Source
Escherichia Coli.
Synonyms
Neutrophil cytosol factor 4, NCF-4, Neutrophil NADPH oxidase factor 4, SH3 and PX domain-containing protein 4, p40-phox, p40phox, NCF4, SH3PXD4, NCF, SH3PXD4.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NCF4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 359 amino acids (1-339 a.a) and having a molecular mass of 41.1kDa.
NCF4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Neutrophil Cytosolic Factor 4 (NCF4) is a key regulatory component of the NADPH oxidase enzyme system in phagocytes, crucial for the host's immune defense. Primarily found in myeloid cells, NCF4 forms a complex with NCF2, which interacts with NCF1 and the RAC1 protein. This complex activates flavocytochrome b, a critical part of the NADPH oxidase system. NCF4 also plays a role in PI(3) kinase signaling pathways. Its phosphorylation can downregulate enzyme activity.
Description
Recombinant human NCF4, expressed in E. coli, is a purified, non-glycosylated polypeptide chain consisting of 359 amino acids (with the first 339 being the native sequence) and a molecular weight of 41.1 kDa. The protein includes an N-terminal 20 amino acid His-tag for purification.
Physical Appearance
A clear, colorless, and sterile-filtered solution.
Formulation
The NCF4 protein solution is provided at a concentration of 1 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), the product should be kept at 4°C. For longer periods, storage at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advised for extended storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Neutrophil cytosol factor 4, NCF-4, Neutrophil NADPH oxidase factor 4, SH3 and PX domain-containing protein 4, p40-phox, p40phox, NCF4, SH3PXD4, NCF, SH3PXD4.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAVAQQLRAE SDFEQLPDDV AISANIADIE EKRGFTSHFV FVIEVKTKGG SKYLIYRRYR QFHALQSKLE ERFGPDSKSS ALACTLPTLP AKVYVGVKQE IAEMRIPALN AYMKSLLSLP VWVLMDEDVR IFFYQSPYDS EQVPQALRRL RPRTRKVKSV SPQGNSVDRM AAPRAEALFD FTGNSKLELN FKAGDVIFLL SRINKDWLEG TVRGATGIFP LSFVKILKDF PEEDDPTNWL RCYYYEDTIS TIKDIAVEED LSSTPLLKDL LELTRREFQR EDIALNYRDA EGDLVRLLSD EDVALMVRQA RGLPSQKRLF PWKLHITQKD NYRVYNTMP.

Product Science Overview

Gene and Protein Structure

The NCF4 gene is located on chromosome 22 and encodes a protein that is approximately 40 kilodaltons in size . The protein contains several important domains, including a PX domain that binds to phospholipid products of phosphoinositide 3-kinase (PI3K), suggesting its role in PI3K-mediated signaling events . The protein also has an SH3 domain, which is involved in protein-protein interactions .

Function and Mechanism

Neutrophil Cytosolic Factor 4 is primarily expressed in cells of the myeloid lineage, such as neutrophils and macrophages . It interacts with other components of the NADPH oxidase complex, including Neutrophil Cytosolic Factor 1 (p47-phox) and Neutrophil Cytosolic Factor 2 (p67-phox), to form a functional enzyme complex . Upon activation, this complex translocates to the membrane and interacts with flavocytochrome b, the catalytic core of the enzyme system, to produce superoxide anions .

Clinical Significance

Mutations in the NCF4 gene can lead to chronic granulomatous disease, a condition characterized by the inability of phagocytes to produce reactive oxygen species, resulting in recurrent infections . Additionally, Neutrophil Cytosolic Factor 4 has been implicated in various inflammatory diseases due to its role in the production of reactive oxygen species .

Research and Applications

Recombinant forms of Neutrophil Cytosolic Factor 4 are used in research to study the function of the NADPH oxidase complex and its role in the immune response . These studies are crucial for understanding the molecular mechanisms underlying immune system disorders and for developing potential therapeutic interventions .

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Source
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