NCEH1 Human

The NCEH1 gene is located on chromosome 3q26.31 in humans . The enzyme itself is a member of the serine hydrolase family and exhibits carboxylic ester hydrolase activity . It is involved in the hydrolysis of 2-acetyl monoalkylglycerol ether, a precursor in the synthesis of platelet-activating factor and lysophospholipids .

NCEH1 is responsible for the hydrolysis of cholesterol esters in macrophages, contributing to the regulation of cholesterol homeostasis and the development of atherosclerosis . The enzyme also plays a role in the metabolism of xenobiotics and low-density lipoprotein (LDL) particle clearance .

Research has shown that NCEH1 is highly expressed in certain cancer cell lines, suggesting a potential role in cancer development . Reduction of NCEH1 levels in cancer cells has been associated with decreased tumor migration and growth in mice . Additionally, NCEH1 can hydrolyze organophosphates, such as the pesticide metabolite chlorpyrifos oxon .

Recombinant NCEH1 is produced using recombinant DNA technology, which involves inserting the NCEH1 gene into a suitable expression system, such as bacteria or yeast, to produce the enzyme in large quantities. This recombinant enzyme is used in various research applications to study its function and potential therapeutic uses.

Recent studies have highlighted the role of NCEH1 in endothelial function, particularly in the context of diabetes . Deficiency of NCEH1 has been shown to impair endothelial function in diet-induced diabetic mice, suggesting that NCEH1 may be a promising candidate for the prevention and treatment of vascular complications associated with diabetes .