NCALD Human

Neurocalcin Delta Recombinant Human
Cat. No.
BT27795
Source
Escherichia Coli.
Synonyms
Rocalcin Delta, MGC33870, MGC74858, Neurocalcin-Delta.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NCALD produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (1-193.a.a) and having a molecular mass of 24.4kDa.
NCALD is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
NCALD, a member of the neuronal calcium sensor (NCS) family, plays a crucial role in calcium-mediated signal transduction. In its inactive state (low intracellular calcium levels), NCALD resides in the cytoplasm. However, elevated calcium levels trigger a conformational change, exposing its myristoyl group. This exposure facilitates membrane association and partial co-localization with the perinuclear trans-golgi network. NCALD functions as a regulator of signal transduction pathways initiated by G protein-coupled receptors.
Description
This product consists of non-glycosylated, recombinant human NCALD protein. Expressed in E. coli, it presents as a single polypeptide chain with a molecular weight of 24.4 kDa. The protein encompasses 213 amino acids, including a 20-amino acid His-tag fused at the N-terminus (1-193 a.a). Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear, sterile-filtered solution.
Formulation
The NCALD protein solution is provided at a concentration of 0.5 mg/ml in a buffer composed of 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, and 40% glycerol.
Stability
For short-term storage (2-4 weeks), maintain the product at 4°C. For extended storage, freezing at -20°C is recommended. To enhance stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Minimize freeze-thaw cycles.
Purity
The purity of the NCALD protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Rocalcin Delta, MGC33870, MGC74858, Neurocalcin-Delta.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF.

Product Science Overview

Structure and Function

Neurocalcin Delta contains several key structural features:

  • N-terminal myristoylation signal: This signal allows the protein to attach to cell membranes.
  • Four EF-hand calcium-binding loops: These loops enable the protein to bind calcium ions .

At resting calcium levels, Neurocalcin Delta is primarily cytosolic. However, when intracellular calcium levels rise, the protein undergoes a conformational change that exposes the myristoyl group, leading to its association with membranes and partial co-localization with the perinuclear trans-Golgi network . This calcium-dependent translocation is essential for its role in cellular signaling.

Biological Role

Neurocalcin Delta is involved in several critical cellular functions:

  • Regulation of G protein-coupled receptor (GPCR) signal transduction: It modulates the activity of GPCRs, which are involved in various physiological processes .
  • Calcium-dependent regulation of rhodopsin phosphorylation: This process is vital for visual signal transduction .
Expression and Localization

Neurocalcin Delta is predominantly expressed in the brain, where it plays a significant role in neuronal function . Its expression is tightly regulated, and it is involved in the transmission across chemical synapses and the presynaptic function of kainate receptors .

Recombinant Production

Recombinant Neurocalcin Delta is typically produced in Escherichia coli (E. coli) systems. This method allows for the efficient production of the protein for research and therapeutic purposes . The recombinant protein retains its functional properties, making it a valuable tool for studying calcium signaling and related pathways.

Clinical Relevance

Mutations or dysregulation of the NCALD gene have been associated with certain diseases, including seminal vesicle cystadenoma and seminal vesicle tumor . Understanding the role of Neurocalcin Delta in these conditions could provide insights into potential therapeutic targets.

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