NAT6 Antibody

N-Acetyltransferase 6, Mouse Anti Human
Cat. No.
BT21302
Source
Synonyms
Protein fusion-2, FUS2, FUS-2, NAT6, N-acetyltransferase 6, Protein fus-2.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
NAT6, primarily found in the cytoplasm, is an enzyme responsible for transferring acetyl groups from acetyl-CoA to acrylamines. It is known for its ability to acetylate the N-terminus of proteins through a ping-pong-like mechanism and its specific substrate preferences. Notably, the NAT6 gene is located on chromosome 3p21.3, a region associated with tumor suppressor genes, highlighting the significant role NAT6 function plays in cancer development.
Formulation
The antibody is supplied at a concentration of 1mg/ml in a solution of phosphate-buffered saline (PBS) at pH 7.4 with 0.1% sodium azide added as a preservative.
Storage Procedures
For short-term storage (up to 1 month), the antibody should be kept at 4°C. For longer storage, it is recommended to store at -20°C. Repeated freezing and thawing should be avoided to maintain antibody stability.
Stability / Shelf Life
The antibody exhibits stability for 12 months when stored at -20°C and for 1 month when stored at 4°C.
Applications
The NAT6 antibody has undergone rigorous testing using ELISA and Western blot analysis, ensuring its specificity and reactivity. However, optimal working dilutions may vary depending on the specific application. For Western blot and immunofluorescence analyses, a dilution range of 1:500 to 1:1000 is recommended, with an initial starting dilution of 1:500.
Synonyms
Protein fusion-2, FUS2, FUS-2, NAT6, N-acetyltransferase 6, Protein fus-2.
Purification Method
NAT6 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT2F4AT.
Immunogen
Anti-human NAT6 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NAT6 amino acids 1-308 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.

Product Science Overview

Introduction

N-Acetyltransferase 6 (NAT6), also known as FUS-2 or Protein fus-2, is an enzyme that belongs to the GCN5-related N-acetyltransferase (GNAT) family. This enzyme is involved in the acetylation of proteins, a crucial post-translational modification that can affect protein function, stability, and interactions. The mouse anti-human NAT6 antibody is a polyclonal antibody raised in mice against the full-length human NAT6 protein.

Structure and Function

NAT6 is encoded by the NAT6 gene, which is located on chromosome 3 in humans. The enzyme consists of 308 amino acids and has a molecular weight of approximately 34 kDa. NAT6 is known to acetylate the N-terminal residues of various proteins, which can influence their activity and interactions. This modification is essential for regulating protein function and maintaining cellular homeostasis.

Biological Significance

The acetylation activity of NAT6 plays a significant role in various cellular processes, including gene expression, protein stability, and signal transduction. By modifying the N-terminal residues of target proteins, NAT6 can alter their function and interactions, thereby influencing cellular pathways and responses. Dysregulation of NAT6 activity has been implicated in various diseases, including cancer, where abnormal protein acetylation can contribute to tumorigenesis and progression.

Mouse Anti-Human NAT6 Antibody

The mouse anti-human NAT6 antibody is a polyclonal antibody produced by immunizing mice with the full-length human NAT6 protein. This antibody is used in various research applications, including Western blotting, immunoprecipitation, and immunofluorescence, to detect and study the expression and function of NAT6 in human samples. The antibody is available from various suppliers, such as Abnova Corporation and Aviva Systems Biology .

Applications in Research

Researchers use the mouse anti-human NAT6 antibody to investigate the role of NAT6 in different biological processes and diseases. By studying the expression and function of NAT6, scientists can gain insights into its involvement in cellular pathways and its potential as a therapeutic target. The antibody is also used to explore the mechanisms underlying protein acetylation and its impact on cellular function.

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