NAT1 Human

N-Acetyltransferase 1 Human Recombinant
Cat. No.
BT10948
Source
Escherichia Coli.
Synonyms
N-acetyltransferase 1 (arylamine N-acetyltransferase), AAC1, Arylamide acetylase 1, Monomorphic arylamine N-acetyltransferase, MNAT, NAT-1.
Appearance
NAT1 is supplied as a sterile filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NAT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 310 amino acids (1-290) and having a molecular mass of 36.1 kDa.
The NAT1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
NAT1, a member of the arylamine N-acetyltransferase family, catalyzes the N- or O-acetylation of heterocyclic and arylamine substrates, playing a crucial role in drug detoxification. Notably, NAT1 is involved in the metabolism of a wide range of drugs and xenobiotics, contributing to the detoxification of numerous hydrazine and arylamine drugs. Interestingly, certain alleles associated with elevated N-acetyltransferase activity have been linked to an increased risk of colon and urinary bladder cancers, as NAT1 can also bioactivate several known carcinogens.
Description
Recombinant human NAT1, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 310 amino acids (residues 1-290) with a molecular weight of 36.1 kDa. This protein is engineered with a 20 amino acid His-Tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
NAT1 is provided as a clear, sterile solution after filtration.
Formulation
NAT1 protein is supplied at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8), 0.1 M NaCl, 2 mM DTT, and 10% Glycerol.
Stability
For short-term storage (2-4 weeks), NAT1 should be kept at 4°C. For extended storage, it is recommended to store the protein at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Importantly, avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of NAT1 is greater than 85%, as assessed by SDS-PAGE analysis.
Synonyms
N-acetyltransferase 1 (arylamine N-acetyltransferase), AAC1, Arylamide acetylase 1, Monomorphic arylamine N-acetyltransferase, MNAT, NAT-1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MDIEAYLERI GYKKSRNKLD LETLTDILQH QIRAVPFENL NIHCGDAMDL GLEAIFDQVV RRNRGGWCLQ VNHLLYWALT TIGFETTMLG GYVYSTPAKK YSTGMIHLLL QVTIDGRNYI VDAGFGRSYQ MWQPLELISG KDQPQVPCIF RLTEENGFWY LDQIRREQYI PNEEFLHSDL LEDSKYRKIY SFTLKPRTIE DFESMNTYLQ TSPASVFTSK SFCSLQTPDG VHCLVGFTLT HRRFNYKDNT DLIEFKTLSE EEIEKVLKNI FNISLQRKLV PKHGDRFFTI

Product Science Overview

Gene and Protein Structure

The NAT1 gene is located on chromosome 8 at the position 8p22 . It is one of the two arylamine N-acetyltransferase genes present in the human genome, with the other being NAT2. The enzyme encoded by NAT1 is orthologous to the mouse and rat Nat2 genes .

Function and Mechanism

NAT1 catalyzes both N- and O-acetylation reactions. These reactions are essential for the detoxification of a wide range of drugs and environmental carcinogens. The enzyme helps in the metabolism of heterocyclic and arylamine substrates, which are often found in various pharmaceuticals and environmental toxins . Additionally, NAT1 is involved in folate catabolism, which is vital for cellular function and DNA synthesis .

Clinical Significance

NAT1 has been implicated in the bioactivation of several known carcinogens. Genetic polymorphisms in the NAT1 gene can lead to variations in enzyme activity, which may influence an individual’s susceptibility to certain cancers, such as colon and urinary bladder cancers . High levels of NAT1 activity have been linked to an increased risk of these cancers due to the enzyme’s role in activating carcinogenic compounds .

Recombinant NAT1

Recombinant NAT1 refers to the enzyme produced through recombinant DNA technology. This involves inserting the NAT1 gene into a suitable expression system, such as bacteria or yeast, to produce the enzyme in large quantities. Recombinant NAT1 is used in various research applications to study its function, mechanism, and role in drug metabolism and carcinogenesis .

Expression and Localization

NAT1 is predominantly expressed in the cytoplasm and cytosol of cells . It is highly expressed in tissues such as the bronchial epithelial cells, mucosa of the ileum, colon, and nasopharynx . The enzyme’s expression pattern suggests its significant role in the metabolism of inhaled and ingested xenobiotics.

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