NARS Human, Sf9
Description
NARS is expressed with a -6xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
AsnRS is a cytoplasmic enzyme that catalyzes the attachment of asparagine to its corresponding tRNA (tRNA^Asn). This reaction is vital for the accurate translation of the genetic code into functional proteins. The enzyme recognizes both the amino acid and its corresponding tRNA, ensuring the correct amino acid is incorporated into the growing polypeptide chain.
The human recombinant version of AsnRS, produced in Sf9 cells, is a glycosylated polypeptide chain with a calculated molecular mass of approximately 63,853 Daltons . This recombinant form is often tagged with a His-tag at the N-terminus to facilitate purification.
The recombinant AsnRS is typically expressed in Sf9 cells, a cell line derived from the fall armyworm (Spodoptera frugiperda). These cells are commonly used in biotechnology for the production of recombinant proteins due to their ability to perform post-translational modifications similar to those in higher eukaryotes.
The purification process involves several chromatographic techniques to isolate the enzyme with high purity. The His-tag at the N-terminus allows for affinity purification using nickel or cobalt-based resins, which bind to the histidine residues, enabling the separation of the tagged protein from other cellular components.
Recombinant AsnRS has several applications in research and biotechnology:
- Structural Studies: Understanding the three-dimensional structure of AsnRS can provide insights into its function and mechanism of action. This knowledge is crucial for designing inhibitors that can modulate the enzyme’s activity, which could have therapeutic implications.
- Protein Synthesis: AsnRS is used in in vitro translation systems to study protein synthesis and the role of aminoacyl-tRNA synthetases in this process.
- Drug Development: The enzyme can be a target for developing drugs that inhibit its activity, potentially leading to new treatments for diseases caused by errors in protein synthesis.
The recombinant AsnRS is stable when stored at 4°C for short periods (2-4 weeks) and at -20°C for longer periods. To prevent degradation, it is recommended to avoid multiple freeze-thaw cycles and to add a carrier protein, such as human serum albumin (HSA) or bovine serum albumin (BSA), for long-term storage .
