NAPG Human

N-ethylmaleimide-Sensitive Factor Attachment Protein, Gamma Human Recombinant
Cat. No.
BT27635
Source
Escherichia Coli.
Synonyms
Gamma-soluble NSF attachment protein, SNAP-gamma, N-ethylmaleimide-sensitive factor attachment protein gamma, NAPG, SNAPG, GAMMASNAP.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NAPG Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 336 amino acids (1-312 a.a.) and having a molecular mass of 37.3kDa.
NAPG is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Gamma-SNAP, also known as NAPG, is a cytoplasmic protein that plays a crucial role in membrane fusion reactions. It binds to a membrane receptor complex consisting of VAMP, SNAP25, and syntaxin. This interaction facilitates the membrane binding of NSF, an ATPase essential for membrane fusion. NAPG, along with NSF and other SNAPs, is believed to be a fundamental component of the intracellular membrane fusion machinery. Its specific action at different fusion sites is likely regulated by SNAP receptors unique to the membranes involved. In platelets, NAPG is involved in exocytosis, controlling the membrane fusion events during this process.
Description
Recombinant Human NAPG, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 336 amino acids, with a molecular weight of 37.3 kDa. The protein sequence includes amino acids 1-312 of NAPG and a 24 amino acid His-tag fused at the N-terminus. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
NAPG protein is supplied in a solution at a concentration of 1 mg/ml. The solution contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.1M NaCl, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of NAPG is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Gamma-soluble NSF attachment protein, SNAP-gamma, N-ethylmaleimide-sensitive factor attachment protein gamma, NAPG, SNAPG, GAMMASNAP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAAQKI NEGLEHLAKA EKYLKTGFLK WKPDYDSAAS EYGKAAVAFK NAKQFEQAKD ACLREAVAHE NNRALFHAAK AYEQAGMMLK EMQKLPEAVQ LIEKASMMYL ENGTPDTAAM ALERAGKLIE NVDPEKAVQL YQQTANVFEN EERLRQAVEL LGKASRLLVR GRRFDEAALS IQKEKNIYKE IENYPTCYKK TIAQVLVHLH RNDYVAAERC VRESYSIPGF NGSEDCAALE QLLEGYDQQD QDQVSDVCNS PLFKYMDNDY AKLGLSLVVP GGGIKKKSPA TPQAKPDGVT ATAADEEEDE YSGGLC.

Product Science Overview

Introduction

N-ethylmaleimide-Sensitive Factor (NSF) is a crucial protein involved in various cellular processes, particularly in membrane fusion events. The gamma isoform of the NSF attachment protein, also known as SNAP-γ, plays a significant role in the intracellular trafficking and fusion of vesicles to target membranes.

Structure and Function

NSF is a homohexameric AAA ATPase, which means it forms a hexameric structure and utilizes ATP to drive its functions. It is ubiquitously found in the membranes of eukaryotic cells and is a central component of the cellular machinery responsible for the transfer of membrane vesicles from one compartment to another .

The primary function of NSF is to catalyze the fusion of transport vesicles within the Golgi apparatus and between the endoplasmic reticulum and the Golgi stack. This process is essential for the delivery of cargo proteins to various compartments within the cell .

Mechanism of Action

NSF operates in conjunction with soluble NSF attachment proteins (SNAPs) and SNARE proteins. SNAPs enable NSF to bind to target membranes, while SNARE proteins on two joining membranes form a complex. The α-helical domains of the SNAREs coil around each other, forming a stable four-helix bundle. NSF then uses the energy from ATP hydrolysis to disassemble the SNARE complex, allowing the membranes to fuse .

Biological Significance

NSF and its associated proteins are involved in several critical cellular processes, including:

  • Intracellular Protein Transport: NSF is essential for the transport of proteins between different cellular compartments.
  • Receptor Recycling: NSF plays a role in the positive regulation of receptor recycling, ensuring that receptors are efficiently reused within the cell.
  • Protein Catabolism: NSF is involved in the positive regulation of protein catabolic processes, contributing to the breakdown and recycling of proteins .
Clinical Relevance

Mutations or dysregulation of NSF can lead to various diseases. For instance, NSF has been implicated in developmental and epileptic encephalopathy, a severe neurological disorder. Understanding the function and regulation of NSF is crucial for developing potential therapeutic strategies for such conditions .

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