NAPA Human

N-Ethylmaleimide-Sensitive Factor Attachment Protein, Alpha Human Recombinant
Cat. No.
BT6204
Source
Escherichia Coli.
Synonyms
SNAPA, SNAP-alpha.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NAPA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 315 amino acids (1-295) and having a molecular mass of 35.3 kDa. NAPA is fused to 20 amino acid His Tag at N-terminus and purified by standard chromatography techniques.

Product Specs

Introduction
NAPA, a member of the Soluble NSF Attachment Protein (SNAP) family, plays a crucial role in membrane fusion processes. SNAPs, along with SNAP receptors (SNAREs) and N-ethylmaleimide-sensitive fusion protein (NSF), are essential for fusing transport vesicles with target membranes. This fusion process is vital for various cellular activities, including synaptic transmission (communication between nerve cells), intra-Golgi transport (within the Golgi apparatus), endosome-to-endosome fusion, and the transport of transcytotic vesicles to the plasma membrane. NAPA is specifically responsible for binding to NSF, leading to the formation of a 20S fusion particle, a complex crucial for membrane fusion.
Description
Recombinant human NAPA, expressed in E. coli, is a single polypeptide chain devoid of glycosylation. It comprises 315 amino acids, encompassing residues 1 to 295, and has a molecular weight of 35.3 kDa. A 20 amino acid Histidine tag is attached to the N-terminus of the protein to facilitate purification by standard chromatography techniques.
Physical Appearance
The product is a clear, colorless, and sterile filtered solution.
Formulation
The NAPA protein is supplied in a solution containing 1mg/ml of protein in a buffer composed of 20mM Tris-HCl at pH 7.5 and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. To ensure stability during long-term storage, adding a carrier protein such as 0.1% HSA (human serum albumin) or BSA (bovine serum albumin) is advised. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the NAPA protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
SNAPA, SNAP-alpha.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA
HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR.

Product Science Overview

Introduction

N-Ethylmaleimide-Sensitive Factor Attachment Protein, Alpha (α-SNAP) is a crucial component of the cellular machinery involved in membrane fusion. This protein is encoded by the NAPA gene and is ubiquitously expressed in eukaryotic cells . The recombinant form of this protein is often used in research to study its function and interactions.

Structure and Function

α-SNAP is a soluble protein that plays a pivotal role in the SNARE (Soluble NSF Attachment Protein Receptor) complex . The SNARE complex is essential for the fusion of vesicles with target membranes, a process critical for intracellular trafficking and neurotransmitter release . α-SNAP binds to the SNARE complex and recruits the N-ethylmaleimide-sensitive factor (NSF), an ATPase that disassembles the SNARE complex after membrane fusion .

Mechanism of Action

The primary function of α-SNAP is to mediate the disassembly of the SNARE complex. This process is ATP-dependent and involves the hydrolysis of ATP by NSF . The binding of α-SNAP to the SNARE complex is a prerequisite for NSF attachment and subsequent ATP hydrolysis . This disassembly is crucial for the recycling of SNARE proteins and the maintenance of vesicular trafficking .

Biological Significance

α-SNAP is indispensable for various cellular processes, including synaptic transmission, hormone secretion, and vesicular transport . Alterations in α-SNAP expression or function have been linked to several neurological disorders, highlighting its importance in maintaining cellular homeostasis .

Research and Applications

Recombinant α-SNAP is widely used in biochemical and structural studies to understand its role in membrane fusion and SNARE complex dynamics . It is also employed in the development of therapeutic strategies targeting SNARE-mediated processes in diseases such as epilepsy and neurodegenerative disorders .

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