NAP 2 Rat
Neutrophil Activating Protein-2 Rat Recombinant (CXCL7)
Cat. No.
BT22140
Source
Escherichia Coli.
Synonyms
Platelet basic protein, PBP, Small inducible cytokine B7, CXCL7, Leukocyte-derived growth factor, LDGF, Macrophage-derived growth factor, MDGF, pro-platelet basic protein (chemokine (C-X-C motif) ligand 7), TC1, TC2, TGB, TGB1, B-TG1, CTAP3, NAP-2, SCYB7, THBGB, LA-PF4, THBGB1, Beta-TG, CTAPIII, CTAP-III.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
NAP-2 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 62 amino acids and having a molecular mass of 6.8kDa.
The NAP 2 is purified by proprietary chromatographic techniques.
The NAP 2 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Chemokine (C-X-C motif) ligand (CXCL7), also known as neutrophil-activating peptide 2 (NAP-2), is a small cytokine classified as a CXC chemokine. This protein is released in significant quantities from activated platelets and plays a role in various cellular processes, including stimulating cell division (mitogenesis), promoting the production of extracellular matrix, influencing glucose metabolism, and contributing to the synthesis of plasminogen activator.
Description
NAP-2 Rat Recombinant, produced in E.coli, is a single-chain polypeptide that lacks glycosylation. It consists of 62 amino acids, resulting in a molecular weight of 6.8kDa. The purification of NAP-2 is achieved through proprietary chromatographic techniques.
Physical Appearance
The product appears as a sterile, white powder that has been lyophilized (freeze-dried).
Formulation
The NAP-2 protein was lyophilized from a 0.2µm filtered solution concentrated in a 1x phosphate-buffered saline (PBS) at a pH of 7.4.
Solubility
To reconstitute the lyophilized NAP-2, it is recommended to dissolve it in sterile 18 megaohm-centimeter (MΩ·cm) H₂O at a concentration of at least 100µg/ml. This solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized NAP-2, while stable at room temperature for up to 3 weeks, should be stored in a dry environment below -18°C. After reconstitution, CXCL7 should be stored at 4°C for a period of 2 to 7 days. For long-term storage, freezing below -18°C is recommended. It is advisable to add a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), for long-term storage and to avoid repeated freezing and thawing cycles.
Purity
The purity of the product is determined to be greater than 97.0% through the following analyses: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) and (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Biological Activity
The biological activity of NAP-2 is assessed by its chemoattractant properties on BaF3 mouse pro-B cells expressing human CXCR2. The half-maximal effective concentration (ED₅₀) for this effect is observed to be less than 10ng/ml, which corresponds to a specific activity of 100,000 units per milligram.
Synonyms
Platelet basic protein, PBP, Small inducible cytokine B7, CXCL7, Leukocyte-derived growth factor, LDGF, Macrophage-derived growth factor, MDGF, pro-platelet basic protein (chemokine (C-X-C motif) ligand 7), TC1, TC2, TGB, TGB1, B-TG1, CTAP3, NAP-2, SCYB7, THBGB, LA-PF4, THBGB1, Beta-TG, CTAPIII, CTAP-III.
Source
Escherichia Coli.
Amino Acid Sequence
IELRCRCTNT LSGIPLNSIS RVNVFRPGAH CDNVEVIATL KNGKEVCLDP TAPMIKKIVK KI.
Product Science Overview
Structure and Origin
Biological Functions
NAP-2 is known for its ability to stimulate a variety of cellular processes, including:
- Chemoattraction and Activation of Neutrophils: NAP-2 binds to the CXCR2 receptor on neutrophils, leading to their activation and migration towards sites of inflammation .
- Degranulation: It stimulates the release of inflammatory mediators and degradative enzymes such as serine proteases and lysozymes from neutrophils .
- Angiogenesis: NAP-2 has been shown to induce endothelial cell proliferation and chemotaxis, which are essential for the formation of new blood vessels .
- Mitosis and Extracellular Matrix Production: It also promotes mitosis and the synthesis of extracellular matrix components .
Role in Disease and Therapeutic Potential
NAP-2’s involvement in various physiological and pathological processes makes it a significant target for research and potential therapeutic interventions:
- Cancer: NAP-2’s role in angiogenesis and cell proliferation makes it a potential target for cancer treatment strategies. It has been associated with tumor growth, survival, invasion, and metastasis .
- Inflammatory Diseases: Due to its role in neutrophil activation and migration, NAP-2 is also implicated in various inflammatory conditions .
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