Introduction
NANS, a 359 amino acid protein, plays a crucial role in sialic acid biosynthesis. This ubiquitously expressed protein contains an AFP (antifreeze proteins)-like domain. NANS catalyzes the water-dependent formation of two key sialic acids: N-acetylneuraminic acid (Neu5Ac) using N-acetylmannosamine 6-phosphate as a substrate and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN) using mannose 6-phosphate as a substrate.
Formulation
The antibody is supplied in a solution containing 1mg/ml of antibody in PBS buffer at pH 7.4, with 0.1% sodium azide added as a preservative.
Storage Procedures
For short-term storage (up to 1 month), store at 4°C. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles to preserve antibody stability.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored at -20°C and 1 month when stored at 4°C.
Applications
This NANS antibody has undergone rigorous testing and validation through ELISA and Western blot analysis, confirming its specificity and reactivity. While the recommended dilution for Western blot analysis is 1:1,000, optimal working concentrations may vary depending on the specific application and should be determined through titration.
Synonyms
Sialic acid synthase, N-acetylneuraminate synthase, N-acetylneuraminate-9-phosphate synthase, N-acetylneuraminic acid phosphate synthase, N-acetylneuraminic acid synthase, NANS, SAS.
Purification Method
NANS antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Immunogen
Anti-human NANS mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NANS amino acids 1-359 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.
