NANA E.Coli

N-Acetylneuraminate Lyase E.Coli Recombinant
Cat. No.
BT7205
Source
Escherichia Coli.
Synonyms
N-acetylneuraminate lyase, N-acetylneuraminate pyruvate-lyase, N-acetylneuraminic acid aldolase, NALase, Sialate lyase, Sialic acid aldolase, Sialic acid lyase, nanA, npl, b3225, JW3194.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

NANA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 317 amino acids (1-297 a.a.) and having a molecular mass of 34.7kDa.
NANA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
N-acetylneuraminate lyase (NanA), an enzyme belonging to the oxo-acid-lyase family, plays a crucial role in cleaving carbon-carbon bonds. Specifically, NanA catalyzes the breakdown of N-acetylneuraminic acid (sialic acid) into pyruvate and N-acetyl-D-mannosamine. Its classification as a Class I aldolase is supported by its inhibition upon reduction with NaBH₄ in the presence of its substrate, a characteristic of Schiff-base-forming aldolases. NanA's activity is significantly hampered by Cu²⁺ ions, p-chloromercuribenzoate, and N-bromosuccinimide. Furthermore, its activity is competitively inhibited by pyruvate, the product of the reaction it catalyzes, as well as structurally similar compounds like dihydroxyacetone and DL-glyceraldehyde.
Description
Produced in E. coli, NANA is a single, non-glycosylated polypeptide chain consisting of 317 amino acids, with the first 297 amino acids forming the NANA protein. With a molecular weight of 34.7kDa, it includes a 20 amino acid His-tag fused at the N-terminus. The purification process involves proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The NANA protein solution is prepared at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0) and 20% glycerol.
Stability
For optimal storage, the product should be kept at 4°C if it will be used entirely within 2-4 weeks. For prolonged storage, freezing at -20°C is recommended. To further enhance long-term stability, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freezing and thawing cycles should be avoided.
Purity
SDS-PAGE analysis indicates a purity greater than 95%.
Synonyms
N-acetylneuraminate lyase, N-acetylneuraminate pyruvate-lyase, N-acetylneuraminic acid aldolase, NALase, Sialate lyase, Sialic acid aldolase, Sialic acid lyase, nanA, npl, b3225, JW3194.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MATNLRGVMA ALLTPFDQQQ ALDKASLRRL VQFNIQQGID GLYVGGSTGE AFVQSLSERE QVLEIVAEEA KGKIKLIAHV GCVSTAESQQ LAASAKRYGF DAVSAVTPFY YPFSFEEHCD HYRAIIDSAD GLPMVVYNIP ALSGVKLTLD QINTLVTLPG
VGALKQTSGD LYQMEQIRRE HPDLVLYNGY DEIFASGLLA GADGGIGSTY NIMGWRYQGI VKALKEGDIQ TAQKLQTECN KVIDLLIKTG VFRGLKTVLH YMDVVSVPLC RKPFGPVDEK YLPELKALAQ QLMQERG.

Product Science Overview

Introduction

N-Acetylneuraminate lyase (NAL), also known as sialic acid aldolase, is an enzyme that catalyzes the reversible cleavage of N-acetylneuraminic acid (Neu5Ac) into N-acetyl-D-mannosamine (ManNAc) and pyruvate . This enzyme is part of the lyase family, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds . The recombinant form of this enzyme, produced in Escherichia coli (E. coli), has significant applications in biochemical research and industrial processes.

Function and Mechanism

NAL plays a crucial role in the metabolism of sialic acids, which are essential components of glycoproteins and glycolipids in many organisms . The enzyme’s activity involves the formation of a Schiff base intermediate, facilitating the aldol cleavage of Neu5Ac . This reaction is reversible, allowing NAL to also catalyze the synthesis of Neu5Ac from ManNAc and pyruvate .

Production in E. Coli

Recombinant protein production in E. coli is a widely used method due to the bacterium’s well-characterized genetics, rapid growth, and high-yield production capabilities . The gene encoding NAL is cloned into an expression vector, which is then introduced into E. coli cells. Upon induction, the recombinant E. coli produces NAL, which can be purified using various chromatographic techniques .

Applications
  1. Biochemical Research: NAL is used in the study of sialic acid metabolism and its role in various biological processes. Its ability to catalyze both the cleavage and synthesis of Neu5Ac makes it a valuable tool for investigating sialic acid pathways .
  2. Industrial Production: The enzyme is employed in the production of sialic acids, which are high-value sugars used in pharmaceuticals and biotechnology . Recombinant NAL from E. coli is particularly useful due to its high activity and stability .
  3. Diagnostic Tools: NAL’s binding ability is utilized in functional assays such as ELISA (Enzyme-Linked Immunosorbent Assay), Western Blotting (WB), and Immunoprecipitation (IP) .
Structural Features

The recombinant NAL produced in E. coli typically includes a His-tag to facilitate purification. The enzyme consists of 297 amino acids and has a molecular weight of approximately 34.7 kDa . The His-tagged version allows for easy purification using nickel-affinity chromatography .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

NPL Human
N-acetylneuraminate Pyruvate Lyase Human Recombinant
Cat. No.
BT7289
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.