NAGK Human
Description
NAGK is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
NAGK is involved in both the de novo and salvage pathways of amino sugar metabolism . In the de novo pathway, GlcNAc is synthesized starting from glycolysis at fructose 6-phosphate . In the salvage pathway, NAGK reutilizes GlcNAc from nutritional sources or lysosomal degradation of oligosaccharides . The phosphorylation of GlcNAc by NAGK is the initial step in the salvage pathway, leading to the formation of GlcNAc-6-phosphate .
GlcNAc-6-phosphate can then enter various metabolic pathways:
- Anabolic Pathway: It leads to the formation of UDP-GlcNAc, which is a substrate for GlcNAc transferases involved in complex oligosaccharide synthesis and intracellular O-GlcNAc formation .
- Catabolic Pathway: It links hexosamine metabolism with the glycolytic pathway, resulting in the formation of fructose 6-phosphate .
- Sialic Acid Biosynthesis: It can be further metabolized to N-acetylneuraminic acid, a key component in sialic acid biosynthesis .
NAGK is a prominent salvage enzyme in amino sugar metabolism in mammals . It has been shown to interact with other proteins such as STK16 and LNX1 and also exhibits ManNAc kinase activity . The enzyme’s activity is essential for maintaining the balance of GlcNAc levels in the body, which is crucial for various cellular functions, including glycoprotein metabolism and cartilage repair .
Recombinant human NAGK is produced using genetic engineering techniques to express the human NAGK gene in a host organism, such as bacteria or yeast. This allows for the production of large quantities of the enzyme for research and therapeutic purposes. Recombinant NAGK is used in various biochemical studies to understand its function and role in amino sugar metabolism .
