NAE1 Human

NEDD8 Activating Enzyme E1 Subunit 1 Human Recombinant
Cat. No.
BT25768
Source
Escherichia Coli.
Synonyms
NEDD8-activating enzyme E1 regulatory subunit, Amyloid beta precursor protein-binding protein 1 59 kDa, APP-BP1, Amyloid protein-binding protein 1, Proto-oncogene protein 1, NAE1, APPBP1, HPP1, ula-1, A-116A10.1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NAE1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 557 amino acids (1-534) and having a molecular mass of 62.7kDa.
NAE1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The NEDD8-activating enzyme E1 regulatory subunit (NAE1) is a member of the ubiquitin-activating E1 family and plays a crucial role in the NEDDylation pathway. This pathway involves the ubiquitin-like molecule NEDD8 and is essential for cell cycle regulation, particularly progression through the S/M checkpoint. NAE1 binds to the beta-amyloid precursor protein, a cell surface protein with signal-transducing properties implicated in Alzheimer's disease.
Description
Recombinant human NAE1, expressed in E.coli, is a non-glycosylated polypeptide chain. This single chain comprises 557 amino acids (residues 1-534), resulting in a molecular weight of 62.7kDa. The protein is engineered with a 23 amino acid His-tag at the N-terminus to facilitate purification, which is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile solution after filtration.
Formulation
The provided NAE1 solution has a concentration of 0.5mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 2mM DTT, 10% glycerol, and 200mM NaCl.
Stability
For short-term storage (2-4 weeks), the NAE1 solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). It is important to avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the NAE1 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
NEDD8-activating enzyme E1 regulatory subunit, Amyloid beta precursor protein-binding protein 1 59 kDa, APP-BP1, Amyloid protein-binding protein 1, Proto-oncogene protein 1, NAE1, APPBP1, HPP1, ula-1, A-116A10.1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAQLGKL LKEQKYDRQL RLWGDHGQEA LESAHVCLIN ATATGTEILK NLVLPGIGSF TIIDGNQVSG EDAGNNFFLQ RSSIGKNRAE AAMEFLQELN SDVSGSFVEE SPENLLDNDP SFFCRFTVVV ATQLPESTSL RLADVLWNSQ IPLLICRTYG LVGYMRIIIK EHPVIESHPD NALEDLRLDK PFPELREHFQ SYDLDHMEKK DHSHTPWIVI IAKYLAQWYS ETNGRIPKTY KEKEDFRDLI RQGILKNENG APEDEENFEE AIKNVNTALN TTQIPSSIED IFNDDRCINI TKQTPSFWIL ARALKEFVAK EGQGNLPVRG TIPDMIADSG KYIKLQNVYR EKAKKDAAAV GNHVAKLLQS IGQAPESISE KELKLLCSNS AFLRVVRCRS LAEEYGLDTI NKDEIISSMD NPDNEIVLYL MLRAVDRFHK QQGRYPGVSN YQVEEDIGKL KSCLTGFLQE YGLSVMVKDD YVHEFCRYGA AEPHTIAAFL GGAAAQEVIK IITKQFVIFN NTYIYSGMSQ TSATFQL.

Product Science Overview

Introduction

NEDD8 Activating Enzyme E1 Subunit 1, also known as NAE1, is a crucial component in the process of neddylation, a post-translational modification similar to ubiquitination. This enzyme plays a significant role in the regulation of various cellular processes, including cell cycle progression, signal transduction, and protein degradation.

Gene and Protein Structure

The NAE1 gene is located on chromosome 16q22.1 and encodes a protein that is approximately 59 kDa in size . The protein is composed of 534 amino acids and has several domains that are essential for its function. NAE1 forms a heterodimer with UBA3, another subunit of the NEDD8-activating enzyme, to create the functional E1 enzyme complex .

Function and Mechanism

NAE1 is responsible for the activation of NEDD8, a ubiquitin-like protein. The activation process involves several steps:

  1. Adenylation: NAE1 first adenylates the C-terminal glycine residue of NEDD8 using ATP.
  2. Thioester Bond Formation: The adenylated NEDD8 is then linked to the catalytic cysteine residue of UBA3, forming a NEDD8-UBA3 thioester and releasing AMP.
  3. Transfer to E2 Enzyme: Finally, NEDD8 is transferred to the catalytic cysteine of the E2 enzyme, UBE2M .

This activation process is crucial for the subsequent conjugation of NEDD8 to target proteins, a process known as neddylation. Neddylation regulates the activity of cullin-RING ligases (CRLs), which are involved in the ubiquitin-proteasome system for protein degradation .

Biological Significance

NAE1 is essential for cell cycle progression, particularly through the S/M checkpoint. Overexpression of NAE1 can lead to apoptosis due to the deregulation of NEDD8 conjugation . The enzyme is also implicated in various diseases, including neurodevelopmental disorders and Alzheimer’s disease .

Clinical and Research Applications

Recombinant human NAE1 is widely used in research to study the neddylation pathway and its role in cellular processes. It is also a potential target for therapeutic interventions in diseases where neddylation is dysregulated .

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