NABP1 Human
Nucleic Acid Binding Protein 1 Human Recombinant
Cat. No.
BT27368
Source
Escherichia Coli.
Synonyms
Nucleic Acid Binding Protein 1, OBFC2A, Sensor Of
Single-Strand DNA Complex Subunit B2, Single-Stranded DNA-Binding Protein 2, SSB2,
Oligonucleotide/Oligosaccharide-Binding Fold Containing 2A, Nucleic
Acid-Binding Protein 1, Oligonucleotide/Oligosaccharide-Binding Fold-Containing
Protein 2A, Sensor Of SsDNA Subunit B2, SOSS-B2, SOSS Complex Subunit B2, hSSB2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
NABP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (1-204 a.a) and having a molecular mass of 24.8kDa.
NABP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Nucleic acid binding protein 1 is part of the SOSS complex, a multiprotein complex which functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, NABP1 acts as a sensor of single-stranded DNA, binding to it, and in particular to polypyrimidines. The SOSS complex associates with DNA lesions and influences various endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair, and maintenance of genomic stability. NABP1 is essential for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways.
Description
Recombinant human NABP1 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain containing amino acids 1-204 and a 23 amino acid His-tag at the N-terminus. The protein has a molecular mass of 24.8 kDa and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The NABP1 protein solution has a concentration of 0.5 mg/ml and contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol, and 2mM DTT.
Stability
For short-term storage (2-4 weeks), store the protein solution at 4°C. For long-term storage, freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the NABP1 protein is greater than 95.0% as determined by SDS-PAGE.
Synonyms
Nucleic Acid Binding Protein 1, OBFC2A, Sensor Of
Single-Strand DNA Complex Subunit B2, Single-Stranded DNA-Binding Protein 2, SSB2,
Oligonucleotide/Oligosaccharide-Binding Fold Containing 2A, Nucleic
Acid-Binding Protein 1, Oligonucleotide/Oligosaccharide-Binding Fold-Containing
Protein 2A, Sensor Of SsDNA Subunit B2, SOSS-B2, SOSS Complex Subunit B2, hSSB2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMNRVNDP LIFIRDIKPG LKNLNVVFIV LEIGRVTKTK DGHEVRSCKV
ADKTGSITIS VWDEIGGLIQ PGDIIRLTRG YASMWKGCLT LYTGRGGELQ KIGEFCMVYS EVPNFSEPNP
DYRGQQNKGA QSEQKNNSMN SNMGTGTFGP VGNGVHTGPE SREHQFSHAG RSNGRGLINP QLQGTASNQT VMTTISNGRD PRRAFKR.
Product Science Overview
Gene and Protein Structure
NABP1 is located on chromosome 2 at the 2q32.3 band in humans . The gene spans approximately 63,029 base pairs and is expressed in various tissues, including the spleen, blood, and endometrium . The protein itself is a single-stranded DNA (ssDNA)-binding protein, which is essential for maintaining genomic stability .
Function and Mechanism
NABP1 is a component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint . Within the SOSS complex, NABP1 acts as a sensor of single-stranded DNA, particularly binding to polypyrimidines . This complex associates with DNA lesions and influences various endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair, and maintenance of genomic stability .
Biological Significance
Clinical Relevance
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