N6AMT1 Human

N-6 Adenine-Specific DNA Methyltransferase 1 Human Recombinant
Cat. No.
BT10679
Source
E.coli.
Synonyms
N-6 Adenine-Specific DNA Methyltransferase 1 (Putative), N(6)-Adenine-Specific DNA Methyltransferase 1, HemK Methyltransferase Family Member 2, M.HsaHemK2P, C21orf127, HEMK2, Chromosome 21 Open Reading Frame 127, N6-DNA-Methyltransferase, EC 2.1.1.- , PRED28, N6AMT, MTQ2, HemK methyltransferase family member 2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

N6AMT1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 237 amino acids (1-214 a.a) and having a molecular mass of 25.3kDa.
N6AMT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
N-6 Adenine-Specific DNA Methyltransferase 1, also known as N6AMT1, is a member of the methyltransferase family. This enzyme plays a crucial role in methylating release factor 1 during the termination of translation. Additionally, N6AMT1 facilitates the conversion of monomethylarsonous acid, an arsenic metabolite, into the less toxic dimethylarsonic acid.
Description
Recombinant human N6AMT1, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 237 amino acids (1-214 a.a.) with a molecular weight of 25.3 kDa. This protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
N6AMT1 protein is supplied in a solution at a concentration of 0.25 mg/ml. The solution is buffered with phosphate-buffered saline (pH 7.4) and contains 20% glycerol and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of N6AMT1 is determined to be greater than 85% based on SDS-PAGE analysis.
Synonyms
N-6 Adenine-Specific DNA Methyltransferase 1 (Putative), N(6)-Adenine-Specific DNA Methyltransferase 1, HemK Methyltransferase Family Member 2, M.HsaHemK2P, C21orf127, HEMK2, Chromosome 21 Open Reading Frame 127, N6-DNA-Methyltransferase, EC 2.1.1.- , PRED28, N6AMT, MTQ2, HemK methyltransferase family member 2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAGENFA TPFHGHVGRG AFSDVYEPAE DTFLLLNALE AAAAELAGVE ICLEVGSGSG VVSAFLASMI GPQALYMCTD INPEAAACTL ETARCNKVHI QPVITDLVKG LLPRLTEKVD LLVFNPPYVV TPPQEVGSHG IEAAWAGGRN GREVMDRFFP LVPDLLSPRG LFYLVTIKEN NPEEILKIMK TKGLQGTTAL SRQAGQETLS VLKFTKS.

Product Science Overview

Introduction

N-6 Adenine-Specific DNA Methyltransferase 1 (N6AMT1) is a crucial enzyme involved in the methylation of adenine residues in DNA. This enzyme is evolutionarily conserved and is found in a wide range of organisms, from prokaryotes to humans . In humans, N6AMT1 plays a significant role in various biological processes, including cell cycle regulation, protein methylation, and arsenic detoxification .

Gene and Protein Structure

The N6AMT1 gene is located on chromosome 21 (21q21.3) in humans . The gene encodes a protein that is approximately 42 kDa in size and consists of several conserved motifs characteristic of methyltransferases . The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to transfer methyl groups to specific adenine residues in DNA .

Biological Functions
  1. DNA Methylation: N6AMT1 is primarily known for its role in DNA methylation, where it adds a methyl group to the N6 position of adenine residues. This modification can influence gene expression and is involved in various cellular processes .
  2. Protein Methylation: When N6AMT1 forms a complex with TRMT112, it functions as a protein methyltransferase. This complex is involved in the methylation of release factor I during translation termination .
  3. Arsenic Detoxification: N6AMT1 is also involved in the detoxification of arsenic. It converts the toxic arsenic metabolite monomethylarsonous acid to the less toxic dimethylarsonic acid .
Role in Disease

N6AMT1 has been associated with several pathological conditions:

  • Cancer: Altered expression of N6AMT1 has been linked to various cancers. The enzyme’s role in cell cycle regulation and proliferation makes it a potential target for cancer therapy .
  • Neurological Disorders: N6AMT1 has been implicated in neurological malfunctions, although the exact mechanisms are not fully understood .
  • Diabetes: There is evidence suggesting that N6AMT1 may play a role in the development of diabetes .
Research and Applications

Recent studies have highlighted the importance of N6AMT1 in cell cycle regulation. For instance, depletion of N6AMT1 in cells leads to increased cell doubling time and disrupted progression through various phases of the cell cycle . Understanding the functions and regulatory mechanisms of N6AMT1 is essential for developing therapeutic strategies for diseases associated with this enzyme.

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