Myostatin Antibody
Description
Product Specs
YPHTHLVHQA NPRGSAGPCC TPTKMSPINM LYFNGKEQII YGKIPAMVVD RCGCS
Product Science Overview
Myostatin, also known as Growth Differentiation Factor 8 (GDF-8), is a member of the Transforming Growth Factor-beta (TGF-β) superfamily. It plays a crucial role in regulating skeletal muscle growth by inhibiting muscle differentiation and growth. The Myostatin Polyclonal Rabbit Anti-Human Antibody is a valuable tool in research for detecting and studying myostatin in various biological samples.
Myostatin is a secreted protein that negatively regulates muscle growth. It is synthesized as a precursor protein that undergoes proteolytic processing to produce a mature, biologically active form. The mature myostatin protein contains seven conserved cysteine residues, which are characteristic of the TGF-β superfamily. Myostatin is expressed predominantly in skeletal muscle tissues and has been shown to play a role in muscle homeostasis and development.
Polyclonal antibodies are a mixture of immunoglobulin molecules secreted against a specific antigen, each recognizing a different epitope. They are produced by immunizing an animal (in this case, a rabbit) with the target antigen (myostatin). The animal’s immune system generates a diverse array of antibodies, which are then collected from the serum. Polyclonal antibodies are advantageous because they recognize multiple epitopes on the target antigen, increasing the likelihood of successful detection.
The production of the Myostatin Polyclonal Rabbit Anti-Human Antibody involves several steps:
- Immunization: Rabbits are immunized with a synthetic peptide corresponding to a specific region of the human myostatin protein. This peptide is often conjugated to a carrier protein, such as Keyhole Limpet Hemocyanin (KLH), to enhance the immune response.
- Serum Collection: After a series of booster immunizations, blood is collected from the rabbits. The serum, which contains the polyclonal antibodies, is separated from the blood cells.
- Purification: The antibodies are purified from the serum using techniques such as affinity chromatography. This process ensures that the final product is highly specific to myostatin and free from other serum proteins.
The Myostatin Polyclonal Rabbit Anti-Human Antibody is used in various research applications, including:
- Western Blotting: To detect myostatin protein levels in tissue and cell lysates.
- Immunohistochemistry: To visualize myostatin expression in tissue sections.
- ELISA: To quantify myostatin levels in biological samples.
- Immunoprecipitation: To isolate myostatin protein complexes from cell lysates.
