MYLPF Human

Myosin Light chain, Phosphorylatable, Fast Skeletal Muscle Human Recombinant
Cat. No.
BT16068
Source
Escherichia Coli.
Synonyms
Myosin regulatory light chain 2 skeletal muscle isoform, Fast skeletal myosin light chain 2, MLC2B, MYLPF, MRLC2, MYL11, HUMMLC2B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MYLPF produced in E.Coli is a single, non-glycosylated polypeptide chain containing 189 amino acids (1-169 a.a) and having a molecular mass of 21.2kDa.
MYLPF is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Myosin regulatory light chains (RLCs) play a crucial role in regulating muscle contraction in both smooth muscle and non-muscle cells. These RLCs, including MYLPF, MRCL3, and MYL9, are regulated through phosphorylation by myosin light chain kinase (MLCK). The phosphorylation process is triggered by calcium and calmodulin and leads to an increase in actin-activated myosin ATPase activity, which is essential for regulating contractile activity. MYLPF, in particular, is vital for the development of both fast and slow skeletal muscles.
Description
The MYLPF protein offered here is produced in E. coli and is a single, non-glycosylated polypeptide chain. It consists of 189 amino acids, including a 20 amino acid His-tag at the N-terminus, and has a molecular weight of 21.2 kDa. The protein is purified using proprietary chromatographic techniques to ensure high purity.
Physical Appearance
The MYLPF protein solution is sterile filtered and colorless.
Formulation
The MYLPF protein is supplied as a solution at a concentration of 0.25 mg/ml. The solution also contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol, and 100mM NaCl.
Stability
For short-term storage (up to 2-4 weeks), the MYLPF protein solution can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. To further enhance stability during long-term storage, adding a carrier protein such as 0.1% HSA or BSA is recommended. Repeated freezing and thawing of the protein should be avoided.
Purity
The purity of the MYLPF protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Myosin regulatory light chain 2 skeletal muscle isoform, Fast skeletal myosin light chain 2, MLC2B, MYLPF, MRLC2, MYL11, HUMMLC2B.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAPKRAKRRT VEGGSSSVFS MFDQTQIQEF KEAFTVIDQN RDGIIDKEDL RDTFAAMGRL NVKNEELDAM MKEASGPINF TVFLTMFGEK LKGADPEDVI TGAFKVLDPE GKGTIKKKFL EELLTTQCDR FSQEEIKNMW AAFPPDVGGN VDYKNICYVI THGDAKDQE.

Product Science Overview

Introduction

Myosin is a fundamental motor protein responsible for muscle contraction in eukaryotic cells. It is composed of two heavy chains and four light chains. The light chains are of two distinct types: phosphorylatable regulatory light chains (MLC2) and nonphosphorylatable alkali light chains (MLC1 and MLC3) . The focus of this article is on the phosphorylatable myosin light chain (MLC2) found in fast skeletal muscle, specifically in its human recombinant form.

Structure and Function

Myosin light chains play a crucial role in modulating the activity of myosin. The phosphorylatable light chain, also known as the regulatory light chain (RLC), is essential for the regulation of muscle contraction. The phosphorylation of RLC is a key mechanism that enhances the interaction between actin and myosin, thereby increasing the force of muscle contraction .

In fast skeletal muscles, the phosphorylation of the myosin light chain is particularly significant. Fast-twitch muscle fibers, which are responsible for rapid and powerful contractions, exhibit a higher rate of myosin light chain phosphorylation compared to slow-twitch fibers. This phosphorylation is mediated by myosin light chain kinase (MLCK), which is activated by calcium ions and calmodulin .

Human Recombinant Myosin Light Chain

The human recombinant form of the phosphorylatable myosin light chain is produced using recombinant DNA technology. This involves inserting the gene encoding the myosin light chain into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. The recombinant protein is then purified for use in various research and clinical applications.

Applications in Research and Medicine

The human recombinant phosphorylatable myosin light chain is widely used in research to study muscle physiology and the mechanisms of muscle contraction. It is also used in the development of therapeutic interventions for muscle-related diseases. For instance, understanding the role of myosin light chain phosphorylation in muscle function can aid in the development of treatments for conditions such as muscular dystrophy and other myopathies .

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