MYL5 Human

Myosin Light Chain 5 (MYL5) is a protein encoded by the MYL5 gene in humans. It is one of the regulatory light chains of myosin, a hexameric ATPase cellular motor protein. Myosin plays a crucial role in muscle contraction and various cellular processes, including intracellular transport and cell motility .

MYL5 is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. The regulatory light chains, including MYL5, are essential for the proper functioning of myosin. They bind calcium ions and participate in the regulation of muscle contraction by modulating the interaction between myosin and actin filaments .

MYL5 is expressed in fetal muscle and in adult tissues such as the retina, cerebellum, and basal ganglia. Its expression pattern suggests a role in both muscle development and the functioning of specific neural tissues .

Recombinant MYL5 is produced using various expression systems, including insect cells. The recombinant protein is typically purified to high levels of purity (>95%) and is suitable for applications such as SDS-PAGE and functional studies .

MYL5 is involved in several critical biological processes. It plays a role in the regulation of muscle contraction, intracellular transport, and cell motility. Additionally, MYL5 is implicated in the regulation of cytoskeletal rearrangements, which are essential for various cellular functions, including cell shape, migration, and division .

Recombinant MYL5 is used in various research applications to study its role in muscle contraction and other cellular processes. It is also utilized in functional assays to investigate the interaction between myosin and actin filaments and to understand the molecular mechanisms underlying muscle contraction and cell motility .