MYL12A Human

Myosin Light Chain 12A Human Recombinant

Cat. No.

BT15221

Source

Escherichia Coli.

Synonyms

Myosin regulatory light chain 12A, MLC-2B, Myosin RLC, Myosin regulatory light chain 2 nonsarcomeric, Myosin regulatory light chain MRLC3, MYL12A, MLCB, MRLC3, RLC, MRCL3, MYL2B.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

MYL12A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 195 amino acids (1-171 a.a.) and having a molecular mass of 22.4kDa.
MYL12A is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Myosin regulatory light chain 12A (MYL12A) is a protein that plays a crucial role in regulating the contraction of both smooth muscle and non-muscle cells. This regulation occurs through a process called phosphorylation. MYL12A is known to be involved in important cellular processes such as cytokinesis, receptor capping, and cell movement.

Description

Recombinant human MYL12A, expressed in E. coli bacteria, is a single polypeptide chain that lacks glycosylation. It comprises 195 amino acids (specifically, amino acids 1 to 171) and has a molecular weight of 22.4 kDa. The protein includes a 24 amino acid His-tag at its N-terminus to aid in purification, which is achieved using proprietary chromatographic methods.

Physical Appearance

A clear and sterile solution.

Formulation

The MYL12A protein is supplied in a solution with a concentration of 0.25 mg/ml. The solution also contains 20mM Tris-HCl buffer at pH 8.0, 20% glycerol, and 1mM DTT.

Stability

For short-term storage (up to 2-4 weeks), the protein solution should be kept at 4°C. For longer storage, it is recommended to freeze the solution at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein (either HSA or BSA) to a final concentration of 0.1%. It is important to avoid repeated freezing and thawing of the protein solution.

Purity

The purity of the MYL12A protein is greater than 90%, as determined by SDS-PAGE analysis.

Synonyms

Myosin regulatory light chain 12A, MLC-2B, Myosin RLC, Myosin regulatory light chain 2 nonsarcomeric, Myosin regulatory light chain MRLC3, MYL12A, MLCB, MRLC3, RLC, MRCL3, MYL2B.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMSSKRT KTKTKKRPQR ATSNVFAMFD QSQIQEFKEA FNMIDQNRDG FIDKEDLHDM LASLGKNPTD EYLDAMMNEA PGPINFTMFL TMFGEKLNGT DPEDVIRNAF ACFDEEATGT IQEDYLRELL TTMGDRFTDE EVDELYREAP IDKKGNFNYI EFTRILKHGA KDKDD.

Product Science Overview

Gene and Protein Information

MYL12A encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and is involved in various cellular processes, including smooth muscle contraction, non-muscle cell contraction, cytokinesis, receptor capping, and cell locomotion. The gene is located on chromosome 18 and has several aliases, including MRLC3, MLCB, and MYL2B.

Structure and Expression

The human recombinant MYL12A protein is produced in E. coli and consists of a single, non-glycosylated polypeptide chain containing 195 amino acids, with a molecular mass of approximately 22.4 kDa. It is fused to a 24 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.

Functional Role

MYL12A is implicated in the regulation of smooth muscle and non-muscle cell contractile activity via its phosphorylation. It is also involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1, which functions as a repressor of p53-driven apoptosis.

Pathways and Interactions

MYL12A is associated with several biological pathways, including:

Semaphorin interactions
Cytoskeleton remodeling
Regulation of actin cytoskeleton by Rho GTPases

Additionally, MYL12A interacts with various proteins and is involved in multiple cellular processes, such as cell proliferation, immune response, and protein ubiquitination.

Clinical Significance

Diseases associated with MYL12A include Glycogen Storage Disease Ib and Myofibrillar Myopathy. The protein’s role in smooth muscle and non-muscle cell contraction makes it a potential target for therapeutic interventions in related disorders.

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MYL12A Human

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