MYD88 Antibody
Myeloid Differentiation Primary Response 88, Mouse Anti Human
Cat. No.
BT26713
Source
Synonyms
Myeloid differentiation primary response protein MyD88, MYD88, MYD88D.
Appearance
Sterile Filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
Myeloid differentiation primary response gene 88 (MYD88) is a cytosolic adapter protein that plays a crucial role in the innate and adaptive immune responses. It acts as a signal transducer in the interleukin-1 and Toll-like receptor signaling pathways, leading to NF-kappa-B activation, cytokine secretion, and inflammation. MYD88 interacts with IRAK1, IRAK2, IRF7, and TRAF6 to mediate these effects. It also increases IL-8 transcription and participates in IL-18-mediated signaling. By activating IRF1, MYD88 promotes its nuclear translocation, leading to the induction of genes such as IFN-beta, NOS2/INOS, and IL12A. Structurally, MYD88 consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Genetic defects in MYD88 are associated with increased susceptibility to pyogenic bacterial infections.
Physical Appearance
Colorless, sterile-filtered solution.
Formulation
The solution contains 1mg/ml of MYD88 Antibody in PBS, pH 7.4, with 10% glycerol and 0.01% sodium azide.
Storage Procedures
For storage up to 1 month, maintain the antibody at 4°C. For longer-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This antibody has been validated for ELISA and Western blot analysis, demonstrating its specificity and reactivity. However, optimal working dilutions should be determined through titration for each specific application. The recommended starting dilution is 1:1000.
Synonyms
Myeloid differentiation primary response protein MyD88, MYD88, MYD88D.
Purification Method
MYD88 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT22F11A.
Immunogen
Anti-human MYD88 mAb, clone PAT22F11A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MYD88 protein 1-309 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and Kappa light chain.
Product Science Overview
Myeloid Differentiation Primary Response 88 (MYD88)
Myeloid Differentiation Primary Response 88 (MYD88) is a crucial adaptor protein in the innate immune system. It was originally discovered in the laboratory of Dan A. Liebermann as a primary response gene involved in myeloid differentiation . The MYD88 gene encodes a protein that plays a pivotal role in the signaling pathways of the immune system, particularly in the activation of immune cells through Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) .
Function and Mechanism:
- Adaptor Protein: MYD88 acts as an adaptor protein that connects proteins receiving signals from outside the cell to those relaying signals inside the cell . This is essential for the proper functioning of the immune response.
- TLR and IL-1R Signaling: MYD88 is involved in the signaling pathways of TLRs and IL-1Rs, which are critical for recognizing pathogens and initiating immune responses . TLRs are pattern recognition receptors (PRRs) that detect pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs) .
- Activation of Transcription Factors: Upon activation by TLRs or IL-1Rs, MYD88 recruits and activates downstream signaling molecules, leading to the activation of transcription factors such as NF-κB and MAPKs . This results in the production of pro-inflammatory cytokines and chemokines, which are essential for the immune response .
Clinical Significance:
- Immune Disorders: Mutations in the MYD88 gene have been associated with various immune disorders, including immunodeficiencies and autoimmune diseases . For example, gain-of-function mutations in MYD88 are linked to certain types of lymphomas .
- Therapeutic Target: Due to its central role in immune signaling, MYD88 is considered a potential therapeutic target for treating inflammatory and autoimmune diseases .
Mouse Anti-Human Antibodies
Mouse anti-Human antibodies are secondary antibodies generated by immunizing mice with human antigens. These antibodies are widely used in research and diagnostic applications due to their specificity and versatility .
Production and Purification:
- Immunization: Mice are immunized with human antigens to produce a robust immune response . The resulting antibodies are then harvested from the mice.
- Purification: The antibodies are purified using techniques such as immunoaffinity chromatography to ensure high specificity and purity .
Applications:
- Detection and Sorting: Mouse anti-Human antibodies are commonly used in various detection methods, including ELISA, Western blotting, and immunohistochemistry . They help in detecting and quantifying human proteins in different samples.
- Signal Amplification: These antibodies can amplify signals in detection assays, increasing the sensitivity and accuracy of the results .
Challenges:
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