MX1 Human

Myxovirus Resistance 1 Human Recombinant

Cat. No.

BT16306

Source

Escherichia Coli.

Synonyms

MX Dynamin-Like GTPase 1, Myxoma Resistance Protein 1, IFI-78K, IFI78, MX, Myxovirus (Influenza) Resistance 1,

Appearance

Sterile Filtered clear solution.

Purity

Greater than 85% as determined by SDS-PAGE.

Usage

THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

MX1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 685 amino acids (1-662 a.a) and having a molecular mass of 77.9kDa.

MX1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Myxovirus Resistance 1, also known as MX1, is a protein involved in the body's defense against viruses. It acts by using a molecule called guanosine triphosphate (GTP) and disrupts the replication of various RNA and DNA viruses. MX1 is activated by signaling molecules like interferon type I and II. Interestingly, there's a similar gene located near MX1 on chromosome 21 and multiple non-functional copies of the gene on chromosome 4.

Description

This product contains the human MX1 protein, which is made in E. coli bacteria. It's a single chain of 685 building blocks called amino acids, with a molecular weight of 77.9kDa. The protein lacks any attached sugar molecules (non-glycosylated) and has a tag of 23 amino acids (His-tag) at one end for purification purposes. We use specific methods to ensure high purity of the MX1 protein.

Physical Appearance

A clear liquid that has been sterilized by filtration.

Formulation

The MX1 protein is provided as a solution (0.25mg/ml) in a special buffer that maintains its stability. The buffer contains phosphate buffered saline (pH7.4), glycerol (10%) and DTT (1mM).

Stability

For short-term storage (up to 4 weeks), keep the product at refrigerated temperature (4°C). For long-term storage, freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for prolonged storage. Avoid repeatedly freezing and thawing the product.

Purity

The purity of the MX1 protein is over 85%, as determined by SDS-PAGE analysis.

Synonyms

MX Dynamin-Like GTPase 1, Myxoma Resistance Protein 1, IFI-78K, IFI78, MX, Myxovirus (Influenza) Resistance 1,

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMVVSEVD IAKADPAAAS HPLLLNGDAT VAQKNPGSVA ENNLCSQYEE KVRPCIDLID SLRALGVEQD LALPAIAVIG DQSSGKSSVL EALSGVALPR GSGIVTRCPL VLKLKKLVNE DKWRGKVSYQ DYEIEISDAS EVEKEINKAQ NAIAGEGMGI SHELITLEIS SRDVPDLTLI DLPGITRVAV GNQPADIGYK IKTLIKKYIQ RQETISLVVV PSNVDIATTE ALSMAQEVDP EGDRTIGILT KPDLVDKGTE DKVVDVVRNL VFHLKKGYMI VKCRGQQEIQ DQLSLSEALQ REKIFFENHP YFRDLLEEGK ATVPCLAEKL TSELITHICK SLPLLENQIK ETHQRITEEL QKYGVDIPED ENEKMFFLID KVNAFNQDIT ALMQGEETVG EEDIRLFTRL RHEFHKWSTI IENNFQEGHK ILSRKIQKFE NQYRGRELPG FVNYRTFETI VKQQIKALEE PAVDMLHTVT DMVRLAFTDV SIKNFEEFFN LHRTAKSKIE DIRAEQEREG EKLIRLHFQM EQIVYCQDQV YRGALQKVRE KELEEEKKKK SWDFGAFQSS SATDSSMEEI FQHLMAYHQE ASKRISSHIP LIIQFFMLQT YGQQLQKAML QLLQDKDTYS WLLKERSDTS DKRKFLKERL ARLTQARRRL AQFPG.

Product Science Overview

Introduction

Myxovirus Resistance 1 (MxA) is a type I interferon-induced GTPase that plays a crucial role in the innate immune response against viral infections. It is part of the dynamin superfamily of large GTPases and is known for its ability to inhibit the replication of a wide range of RNA viruses. The human recombinant form of Myxovirus Resistance 1 (MxA) is produced using recombinant DNA technology, which allows for the expression of the protein in a host organism, typically E. coli.

Structure and Function

MxA is a 77.9 kDa protein composed of 685 amino acids. It contains a GTP-binding domain, which is essential for its antiviral activity. The protein forms oligomers and associates with intracellular membranes, where it exerts its antiviral effects. MxA interferes with the early stages of viral replication by binding to viral nucleocapsids and preventing their transport to the nucleus.

Expression and Regulation

The expression of MxA is tightly regulated by type I interferons (IFNs), such as IFN-α and IFN-β. Upon viral infection, these interferons are produced and bind to their receptors on the cell surface, triggering a signaling cascade that leads to the transcriptional activation of the MxA gene. The protein is then synthesized and accumulates in the cytoplasm, ready to combat viral infections.

Clinical Significance

MxA has been identified as a biomarker for various autoimmune diseases, including systemic lupus erythematosus (SLE) and dermatomyositis. In these conditions, autoantibodies against MxA (anti-MxA) are often present and can be used for diagnostic purposes. Additionally, MxA has been studied for its potential role in interstitial lung disease (ILD) associated with autoimmune myositis.

Recombinant Production

The human recombinant form of MxA is produced using E. coli as the expression system. The protein is expressed as a single, non-glycosylated polypeptide chain and is purified using proprietary chromatographic techniques. The recombinant protein is typically formulated in a phosphate-buffered saline solution containing glycerol and dithiothreitol (DTT) to maintain its stability.

Applications

Recombinant MxA is used in various research applications, including studies on viral replication, immune response, and autoimmune diseases. It serves as a valuable tool for understanding the mechanisms of viral inhibition and the role of MxA in immune regulation.

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MX1 Human

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