MVD Human

Mevalonate Decarboxylase Human Recombinant
Cat. No.
BT5989
Source
E.coli.
Synonyms
Diphosphomevalonate decarboxylase, Mevalonate (diphospho)decarboxylase, MDDase, Mevalonate pyrophosphate decarboxylase, MVD, MPD, FP17780.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MVD Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 420 amino acids (1-400) and having a molecular mass of 45.6kDa.
MVD is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Diphosphomevalonate decarboxylase (MVD) is an enzyme that plays a crucial role in cholesterol biosynthesis. It catalyzes the conversion of mevalonate pyrophosphate to isopentenyl pyrophosphate, an early step in the pathway. This reaction involves decarboxylation and dehydration of the substrate along with ATP hydrolysis. MVD is found in various tissues including the heart, skeletal muscle, lungs, liver, brain, pancreas, kidneys, and placenta.
Description
This product consists of recombinant human MVD, produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 420 amino acids (residues 1-400) and has a molecular weight of 45.6 kDa. The protein includes a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
The MVD solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For longer storage, it is recommended to freeze the product at -20°C. To ensure stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Repeated freezing and thawing should be avoided.
Purity
The purity of this product is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
Diphosphomevalonate decarboxylase, Mevalonate (diphospho)decarboxylase, MDDase, Mevalonate pyrophosphate decarboxylase, MVD, MPD, FP17780.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASEKPLAAV TCTAPVNIAV IKYWGKRDEE LVLPINSSLS VTLHQDQLKT TTTAVISKDF TEDRIWLNGR EEDVGQPRLQ ACLREIRCLA RKRRNSRDGD PLPSSLSCKV HVASVNNFPT AAGLASSAAG YACLAYTLAR VYGVESDLSE VARRGSGSAC RSLYGGFVEW QMGEQADGKD SIARQVAPES HWPELRVLIL VVSAEKKLTG STVGMRASVE TSPLLRFRAE SVVPARMAEM ARCIRERDFP SFAQLTMKDS NQFHATCLDT FPPISYLNAI SWRIIHLVHR FNAHHGDTKV AYTFDAGPNA VIFTLDDTVA EFVAAVWHGF PPGSNGDTFL KGLQVRPAPL SAELQAALAM EPTPGGVKYI IVTQVGPGPQ ILDDPCAHLL GPDGLPKPAA.

Product Science Overview

Introduction

Mevalonate Decarboxylase (MVD), also known as mevalonate diphosphate decarboxylase, is a crucial enzyme in the mevalonate pathway, which is essential for the biosynthesis of isoprenoids. Isoprenoids are a diverse class of biomolecules that include cholesterol, steroid hormones, and other vital compounds. The human recombinant form of this enzyme is produced through recombinant DNA technology, allowing for its study and application in various research and industrial contexts.

The Mevalonate Pathway

The mevalonate pathway, also known as the HMG-CoA reductase pathway, is a metabolic pathway present in eukaryotes, archaea, and some bacteria . This pathway is responsible for the production of isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP), which are the building blocks for isoprenoids . The pathway begins with acetyl-CoA and proceeds through several enzymatic steps to produce these essential compounds.

Role of Mevalonate Decarboxylase

Mevalonate Decarboxylase catalyzes the decarboxylation of mevalonate diphosphate to isopentenyl pyrophosphate (IPP), a key step in the mevalonate pathway . This reaction is crucial for the production of isoprenoids, which are involved in various cellular functions, including membrane integrity, protein prenylation, and energy homeostasis .

Recombinant Production

The human recombinant form of Mevalonate Decarboxylase is produced using recombinant DNA technology. This involves inserting the gene encoding the enzyme into a suitable expression system, such as bacteria or yeast, which then produces the enzyme in large quantities. This recombinant enzyme is used in research to study its structure, function, and role in the mevalonate pathway, as well as in industrial applications for the production of isoprenoids and related compounds.

Applications and Importance
  1. Research: The recombinant enzyme is used to study the mevalonate pathway and its regulation. Understanding this pathway is crucial for developing drugs that target cholesterol biosynthesis, such as statins .
  2. Industrial: The enzyme is used in the production of isoprenoids, which have applications in pharmaceuticals, cosmetics, and other industries .
  3. Medical: Insights gained from studying Mevalonate Decarboxylase can lead to the development of new therapies for diseases related to cholesterol metabolism and other isoprenoid-related disorders .

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