MUG E.Coli

G/U Mismatch-Specific DNA Glycosylase E.Coli Recombinant
Cat. No.
BT22740
Source
E.coli.
Synonyms
xanthine DNA glycosylase, dug, ECK3058, JW3040, ygjF, G/U mismatch-specific DNA glycosylase, Double-strand-specific uracil glycosylase, Mismatch-specific uracil DNA-glycosylase, mug.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MUG Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 191 amino acids (1-168) and having a molecular mass of 21.1kDa. MUG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
As a member of the TDG/mug DNA glycosylase family, G/U mismatch-specific DNA glycosylase (mug) plays a crucial role in repairing DNA damage lesions within stationary-phase cells. This enzyme, Mug, effectively removes three N4-ethenocytosine and excises the uracil base from mismatches, prioritizing U:G over U:A. Following the action of Uracil-N-Glycosylase, which removes uracil from DNA leaving an AP site, Mug further hydrolyzes the carbon-nitrogen bond connecting the DNA's sugar-phosphate backbone and the mispaired base. Notably, the presence of guanine on the complementary strand contributes to substrate recognition.
Description
Recombinantly produced in E.coli, MUG is a single, non-glycosylated polypeptide chain consisting of 191 amino acids (1-168) and possessing a molecular mass of 21.1kDa. A 23 amino acid His-tag is fused to the N-terminus of MUG, which undergoes purification using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The MUG solution (0.5mg/ml) is prepared with the following composition: 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Avoid repeated freezing and thawing of the product.
Purity
The purity of this product exceeds 90% as assessed by SDS-PAGE.
Synonyms
xanthine DNA glycosylase, dug, ECK3058, JW3040, ygjF, G/U mismatch-specific DNA glycosylase, Double-strand-specific uracil glycosylase, Mismatch-specific uracil DNA-glycosylase, mug.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMVEDILA PGLRVVFCGI NPGLSSAGTG FPFAHPANRF WKVIYQAGFT DRQLKPQEAQ HLLDYRCGVT KLVDRPTVQA NEVSKQELHA GGRKLIEKIE DYQPQALAIL GKQAYEQGFS QRGAQWGKQT LTIGSTQIWV LPNPSGLSRV SLEKLVEAYR ELDQALVVRG R.

Product Science Overview

Introduction

G/U mismatch-specific DNA glycosylase, also known as MUG (Mismatch Uracil Glycosylase), is an enzyme found in Escherichia coli (E. coli) that plays a crucial role in the base excision repair (BER) pathway. This enzyme is responsible for identifying and excising uracil and ethenocytosine from DNA, which can arise due to deamination or alkylation of cytosine, respectively .

Structure and Function

The recombinant form of G/U mismatch-specific DNA glycosylase from E. coli is typically expressed in E. coli BL21 (DE3) cells. The enzyme consists of 168 amino acids and has a molecular weight of approximately 19 kDa . The crystal structure of this enzyme reveals a remarkable structural and functional homology to uracil DNA glycosylases (UDGs), despite low sequence identity .

The enzyme operates by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine plays a critical role in substrate recognition, ensuring that the enzyme specifically targets G:U and G:T mismatches .

Biological Significance

G/U mismatch-specific DNA glycosylase is essential for maintaining genomic integrity by repairing promutagenic lesions. These lesions, if left unrepaired, can lead to mutations and genomic instability. The enzyme is particularly important in stationary-phase cells, where DNA damage repair is crucial for cell survival .

Applications

Recombinant G/U mismatch-specific DNA glycosylase from E. coli is widely used in research and biotechnology. It is employed in immunization protocols to generate antibodies that specifically target the protein of interest. The enzyme’s high sensitivity, specificity, and reproducibility make it an ideal tool for various molecular biology applications .

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