MTPN Human

Myotrophin is composed of 118 amino acids and has a molecular weight of approximately 15 kDa . The protein is typically expressed in Escherichia coli (E. coli) for recombinant production . The recombinant form of Myotrophin often includes a His-tag at the N-terminus to facilitate purification and detection .

1. Cardiac Hypertrophy: Myotrophin is known to stimulate protein synthesis and cardiomyocyte growth, which leads to cardiac hypertrophy. This process is mediated through the activation of the NF-kappaB signaling cascade . Elevated levels of Myotrophin have been observed in human dilated cardiomyopathic and ischemic hearts .

2. Cerebellar Morphogenesis: Myotrophin plays a potential role in the development of the cerebellum, particularly in the differentiation of cerebellar neurons such as granule cells .

3. Skeletal Muscle Growth: In addition to its role in cardiac and neural tissues, Myotrophin has been shown to promote skeletal muscle growth both in vitro and in vivo .

Recombinant Myotrophin is widely used in research to study its various functions and potential therapeutic applications. It is utilized in experiments involving SDS-PAGE and mass spectrometry to analyze its purity and molecular weight . The protein is also used in studies focusing on cardiac hypertrophy, neurogenesis, and muscle differentiation.

Recombinant Myotrophin is typically shipped and stored at 4°C for short-term use (1-2 weeks). For long-term storage, it is aliquoted and kept at -20°C or -80°C to avoid freeze-thaw cycles, which can degrade the protein .