MTHSFD Human
Escherichia Coli.
Methenyltetrahydrofolate synthase domain-containing protein, Methenyltetrahydrofolate synthase domaincontaining protein, Methenyltetrahydrofolate synthase
Greater than 90.0% as determined by SDS-PAGE.
Description
MTHSFD Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 406 amino acids (1-383 a.a.) and having a molecular mass of 44.5kDa.
MTHSFD is fused to a 23 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Methenyltetrahydrofolate synthase domain-containing protein, Methenyltetrahydrofolate synthase domaincontaining protein, Methenyltetrahydrofolate synthase
Escherichia Coli.
MGSSHHHHHH SSGLVPRGSH MGSMEPRAVG VSKQDIREQI WGYMESQNLA DFPRPVHHRI PNFKGASHAA EQLPRLQAFK TARTIKVNPD APQKSARFFV LESKKTLLVP TPRLRTGLFN KITPPPGATK DILRKCATSQ GVRNYSVPIG LDSRVLVDLV VVGSVAVSEK GWRIGKGEGY ADLEYAMMVS MGAVSKETPV VTIVHDCQVV DIPEELVEEH DITVDYILTP TRVIATGCKR PKPMGITWFK ISLEMMEKIP ILRSLRAREQ QAGKDVTLQG EHQHLPEPGC QQTVPLSVGR RPPDTPGPET NSMEAAPGSP PGEGAPLAAD VYVGNLPGDA RVSDLKRALR ELGSVPLRLT WQGPRRRAFL HYPDSAAAQQ AVSCLQGLRL GTDTLRVALA RQQRDK
Product Science Overview
Methenyltetrahydrofolate Synthetase Domain Containing (MTHFSD) is a protein-coding gene that plays a crucial role in folate-dependent one-carbon metabolism. This gene is associated with various biological processes and has implications in several diseases. The human recombinant form of this protein is used in research to understand its function and potential therapeutic applications.
The MTHFSD gene is located on chromosome 16 and encodes a protein that contains two significant domains: an RNA recognition motif (RRM) domain and a methenyltetrahydrofolate synthetase (MTHFS) domain . The RRM domain is commonly found in stress granule proteins, while the MTHFS domain is involved in the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, a precursor in folate metabolism .
The primary function of the MTHFSD protein is to facilitate folate-dependent one-carbon metabolism, which is essential for DNA synthesis, repair, and methylation . This process is critical for cell division and growth, making MTHFSD an important protein in rapidly dividing cells, such as those in the bone marrow and gastrointestinal tract.
MTHFSD is expressed in various tissues, including the liver, kidney, and brain . The protein is predominantly localized in the cytoplasm, where it interacts with other components of the folate metabolism pathway . The expression levels of MTHFSD can vary depending on the tissue type and physiological conditions.
Mutations or dysregulation of the MTHFSD gene have been associated with several diseases, including congenital muscular dystrophy-dystroglycanopathy type A8 and mitochondrial complex I deficiency, nuclear type 13 . These conditions highlight the importance of MTHFSD in maintaining cellular function and metabolic balance.
The human recombinant form of MTHFSD is used in research to study its biochemical properties and interactions with other proteins. Understanding the function of MTHFSD can lead to the development of targeted therapies for diseases associated with folate metabolism disorders. Additionally, recombinant MTHFSD can be used in drug screening assays to identify potential inhibitors or activators of the protein.
