Introduction
Metadherin (MTDH) is involved in the downregulation of the SLC1A2/EAAT2 promoter activity when overexpressed. This protein activates the nuclear factor kappa-B (NF-kappa-B) transcription factor and promotes anchorage-independent growth in immortalized melanocytes and astrocytes, a crucial process in tumor cell proliferation. Additionally, MTDH contributes to lung metastasis and potentially influences bone and brain metastasis by enhancing tumor cell attachment to the target organ's endothelium. Metadherin also plays a role in inducing chemoresistance.
Description
Recombinant Human Metadherin, produced in E. coli, is a single, non-glycosylated polypeptide chain (amino acids Val271-Asn456). It comprises 196 amino acids, including a 10 amino acid His tag located at the N-terminus. The calculated molecular mass of the protein is 21.5 kDa.
Physical Appearance
The product appears as a white powder that has been filtered and lyophilized (freeze-dried).
Formulation
The MTDH protein was filtered through a 0.4 μm filter and subsequently lyophilized in a buffer solution containing 20mM Tris and 50mM NaCl at a pH of 7.5.
Solubility
To prepare a working stock solution, it is recommended to add deionized water to the lyophilized pellet, aiming for a concentration of approximately 0.5 mg/ml. Allow sufficient time for the pellet to dissolve completely. Please note that the MTDH protein is not sterile. Prior to using it in cell culture, it is essential to filter the product using an appropriate sterile filter.
Stability
The lyophilized protein should be stored at -20°C. After reconstituting the product, it is advisable to aliquot it to avoid repeated freeze-thaw cycles. The reconstituted protein exhibits stability at 4°C for a limited period; no significant changes were observed after two weeks of storage at this temperature.
Purity
The purity of the protein is greater than 90.0%, as determined by SDS-PAGE analysis.
Synonyms
Metadherin, Lysine-Rich CEACAM1 Co-Isolated Protein, Astrocyte Elevated Gene-1 Protein, Metastasis Adhesion Protein, Astrocyte Elevated Gene 1, 3D3/LYRIC, AEG-1, LYRIC, AEG1, LYRIC/3D3, 3D3, MTDH.
Amino Acid Sequence
MKHHHHHHASVSSGLNENLT VNGGGWNEKS VKLSSQISAG EEKWNSVSPA SAGKRKAEPS AWSQDTGDAN TNGKDWGRSW SDRSIFSGIG STAEPVSQST TSDYQWDVSR NQPYIDDEWS GLNGLSSADP NSDWNAPAEE WGNWVDEERA SLLKSQEPIP DDQKVSDDDK EKGEGALPTG KSKKKKKKKK KQGEDN.
