MSI2 Human

Musashi RNA-Binding Protein 2 Human Recombinant

Cat. No.

BT26029

Source

Escherichia Coli.

Synonyms

MSI2H, RNA-binding protein Musashi homolog 2, Musashi-2, MSI2.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

MSI2 Human Recombinant produced in E. coli is a single polypeptide chain containing 351 amino acids (1-328) and having a molecular mass of 37.6kDa. MSI2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Musashi RNA-Binding Protein 2 (MSI2) is an RNA binding protein with 2 conserved tandem RNA recognition motifs. It plays a role in the proliferation and maintenance of stem cells in the central nervous system. Similar proteins in other species act as RNA-binding proteins and are involved in posttranscriptional gene regulation.

Description

Recombinant human MSI2 protein was produced in E. coli. It is a single polypeptide chain containing 351 amino acids (residues 1-328) with a molecular weight of 37.6kDa. The protein contains a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.

Physical Appearance

Clear, sterile-filtered solution.

Formulation

The MSI2 solution is supplied at a concentration of 0.25mg/ml and is formulated in 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol, and 1mM DTT.

Stability

For short-term storage (up to 4 weeks), store at 4°C. For long-term storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

Purity is determined to be greater than 90% by SDS-PAGE analysis.

Synonyms

MSI2H, RNA-binding protein Musashi homolog 2, Musashi-2, MSI2.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEANGSQ GTSGSANDSQ HDPGKMFIGG LSWQTSPDSL RDYFSKFGEI RECMVMRDPT TKRSRGFGFV TFADPASVDK VLGQPHHELD SKTIDPKVAF PRRAQPKMVT RTKKIFVGGL SANTVVEDVK QYFEQFGKVE DAMLMFDKTT NRHRGFGFVT FENEDVVEKV CEIHFHEINN KMVECKKAQP KEVMFPPGTR GRARGLPYTM DAFMLGMGML GYPNFVATYG RGYPGFAPSY GYQFPGFPAA AYGPVAAAAV AAARGSGSNP ARPGGFPGAN SPGPVADLYG PASQDSGVGN YISAASPQPG SGFGHGIAGP LIATAFTNGY H.

Product Science Overview

Structure and Function

MSI2 contains two conserved tandem RNA recognition motifs (RRMs), which are essential for its function as an RNA-binding protein. These motifs enable MSI2 to bind to specific RNA sequences, thereby regulating the translation of target mRNAs into proteins. This post-transcriptional regulation is vital for controlling gene expression and maintaining cellular homeostasis.

Expression and Localization

MSI2 is expressed in a wide variety of tissues, including neuronal progenitor cells, stem cells, and both normal and leukemic blood cells. It is particularly prominent in the ventricular and subventricular zones of the central nervous system (CNS) during development. In the hematopoietic system, MSI2 is highly expressed in primitive progenitor cells and stem cell compartments.

Role in Development and Disease

MSI2 plays a pivotal role in normal hematopoiesis, the process by which blood cells are formed. It is also involved in the proliferation and maintenance of stem cells in the CNS. The protein’s expression is developmentally regulated, with high levels observed in neural precursor cells and specific neuronal subpopulations.

In addition to its role in normal cellular processes, MSI2 has been implicated in various diseases, including leukemia. Overexpression of MSI2 has been found in myeloid leukemia cell lines, where it contributes to cell proliferation, differentiation, and the maintenance of the cancer stem cell pool.

Research and Therapeutic Potential

Given its critical role in stem cell biology and disease, MSI2 is a target of interest for therapeutic interventions. Researchers are exploring ways to modulate MSI2 activity to treat conditions such as leukemia and other cancers. The human recombinant form of MSI2 is used in various research applications to study its function and potential therapeutic uses.

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MSI2 Human

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