MSH6 Human
Description
Product Specs
Product Science Overview
MSH6 was first identified in the budding yeast Saccharomyces cerevisiae due to its homology with MSH2 . The human MSH6 protein shares a significant sequence similarity with its yeast counterpart, highlighting the evolutionary conservation of this protein . The MSH6 protein contains the Walker-A/B adenine nucleotide binding motif, which is highly conserved across all MutS homologs . This motif is essential for the protein’s ATPase activity, which is crucial for its function in DNA repair .
The primary role of MSH6 is to recognize and initiate the repair of mismatched nucleotides that occur during DNA replication . Mismatches can arise due to replication errors, genetic recombination, or exposure to chemical and physical agents . The MSH6 protein forms a heterodimer with MSH2, known as the hMutS alpha complex . This complex binds to the site of the mismatch and recruits other proteins involved in the repair process .
Mismatch recognition by the hMutS alpha complex is regulated by the transformation of ADP to ATP, which acts as a molecular switch . Once the mismatch is recognized, the complex undergoes a conformational change that facilitates the recruitment of additional repair proteins .
Mutations in the MSH6 gene are associated with hereditary non-polyposis colorectal cancer (HNPCC), also known as Lynch syndrome . This condition is characterized by an increased risk of developing colorectal cancer and other types of cancer at an early age . MSH6 mutations can also lead to endometrial cancer and other malignancies .
Recombinant MSH6 protein is produced using Escherichia coli (E. coli) expression systems . The recombinant protein is typically fused to a GST tag and purified using chromatographic techniques . This recombinant form is used extensively in laboratory research to study the protein’s function and its role in DNA repair .
The recombinant MSH6 protein is supplied as a sterile, filtered solution and is stable for short-term storage at 4°C or long-term storage at -20°C . It is important to avoid freeze-thaw cycles to maintain the protein’s integrity .
