MRTO4 Human

mRNA Turnover 4 Human Recombinant
Cat. No.
BT25853
Source
E.coli.
Synonyms
MRNA Turnover 4 Homolog (S. Cerevisiae), MRT4 MRNA Turnover 4 Homolog (S. Cerevisiae), 60S Acidic Ribosomal Protein PO, Chromosome 1 Open Reading Frame 33, C1orf33, MRT4, DJ657E11.4.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MRTO4 Human Recombinant produced in E. coli is a single polypeptide chain containing 262 amino acids (1-239) and having a molecular mass of 29.9 kDa.
MRTO4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Belonging to the ribosomal protein L10P family, mRNA turnover protein 4 homolog (MRTO4) exhibits a low level of sequence similarity with ribosomal protein P0. While its exact function remains unclear, MRTO4 appears to play a role in mRNA turnover and ribosome assembly. Found in the nucleolus, this protein is part of the ribosome assembly apparatus and shares significant similarity with ribosomal protein P0, competing for binding to the 25S rRNA GAR domain. The gene encoding MRTO4 is situated on human chromosome 1, a chromosome spanning over 260 million base pairs and harboring more than 3,000 genes, representing roughly 8% of the human genome.
Description
Recombinant MRTO4 Human, produced in E. coli, is a single polypeptide chain comprising 262 amino acids (residues 1-239) with a molecular weight of 29.9 kDa. This protein is expressed with an N-terminal 23 amino acid His-tag and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The MRTO4 solution is provided at a concentration of 0.5mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 1mM DTT, and 20% glycerol.
Stability
For optimal storage, the product should be kept at 4°C if it will be used within 2-4 weeks. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. To maintain product integrity, avoid repeated cycles of freezing and thawing.
Purity
The purity of this protein is determined to be greater than 95% based on SDS-PAGE analysis.
Synonyms
MRNA Turnover 4 Homolog (S. Cerevisiae), MRT4 MRNA Turnover 4 Homolog (S. Cerevisiae), 60S Acidic Ribosomal Protein PO, Chromosome 1 Open Reading Frame 33, C1orf33, MRT4, DJ657E11.4.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPKSKRD KKVSLTKTAK KGLELKQNLI EELRKCVDTY KYLFIFSVAN MRNSKLKDIR NAWKHSRMFF GKNKVMMVAL GRSPSDEYKD NLHQVSKRLR GEVGLLFTNR TKEEVNEWFT KYTEMDYARA GNKAAFTVSL DPGPLEQFPH SMEPQLRQLG LPTALKRGVV TLLSDYEVCK EGDVLTPEQA RVLKLFGYEM AEFKVTIKYM WDSQSGRFQQ MGDDLPESAS ESTEESDSED DD

Product Science Overview

Structure and Expression

MRTO4 is a nucleolar component involved in ribosome assembly. It shares significant similarity with the ribosomal protein P0 and competes for binding to the 25S rRNA GAR domain . The human MRTO4 protein consists of 239 amino acids and has a molecular weight of approximately 29.9 kDa . It is typically expressed in E. coli for recombinant protein production and is often tagged with a His-tag for purification purposes .

Function and Mechanism

MRTO4 is primarily involved in the early stages of ribosome assembly. It binds to pre-ribosomal particles and is later replaced by the ribosomal protein P0 during the maturation process . This replacement is crucial for the proper formation of functional ribosomes. MRTO4’s role in mRNA turnover and ribosome assembly makes it an essential protein for maintaining cellular homeostasis and efficient protein synthesis .

Research and Applications

Research on MRTO4 has provided insights into its biophysical characteristics and its role in ribosome assembly. Studies have shown that MRTO4 has a typical α-helix structure and possesses rRNA-binding domains and translation factor binding domains . These structural features are essential for its function in ribosome assembly and mRNA turnover.

Recombinant MRTO4 is used in various research applications, including studies on ribosome assembly, protein synthesis, and cellular homeostasis. The availability of recombinant MRTO4 allows researchers to investigate its interactions with other ribosomal proteins and its role in the overall process of ribosome biogenesis .

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