MRRF Human
Description
Product Specs
MGSSHHHHHH SSGLVPRGSH MATKKAKAKG KGQSQTRVNI NAALVEDIIN LEEVNEEMKS VIEALKDNFN KTLNIRTSPG SLDKIAVVTA DGKLALNQIS QISMKSPQLI LVNMASFPEC TAAAIKAIRE SGMNLNPEVE GTLIRVPIPQ VTREHREMLV KLAKQNTNKA KDSLRKVRTN SMNKLKKSKD TVSEDTIRLI EKQISQMADD TVAELDRHLA VKTKELLG.
Product Science Overview
The Mitochondrial Ribosome Recycling Factor (mtRRF) is a crucial protein involved in the mitochondrial translation process. It plays a significant role in the disassembly of ribosomes from messenger RNA (mRNA) at the termination of mitochondrial protein biosynthesis . This factor is encoded by the MRRF gene and is essential for maintaining mitochondrial function and cellular viability .
The mtRRF is a protein that shares similarities with bacterial ribosome recycling factors due to the evolutionary origin of mitochondria from α-proteobacteria . It functions in collaboration with mitochondrial elongation factor 2 (GFM2) to promote the recycling of mitochondrial ribosomes by dissolving intersubunit contacts . This process is vital for the efficient synthesis of mitochondrial proteins, which are integral components of the oxidative phosphorylation system .
Mitochondria are responsible for producing the majority of cellular ATP through oxidative phosphorylation. The mitochondrial genome encodes 13 essential proteins, along with 22 tRNAs and 2 rRNAs . The synthesis of these proteins occurs on mitoribosomes, which are distinct from cytosolic ribosomes and resemble bacterial ribosomes . The mtRRF ensures the proper recycling of mitoribosomes, preventing their aggregation and maintaining mitochondrial integrity .
The mtRRF binds to the ribosome-mRNA complex at the termination of translation and facilitates the release of the ribosome from the mRNA . This action is crucial for the continuation of protein synthesis cycles within the mitochondria. The recycling process involves the coordinated actions of mtRRF and other mitochondrial translational factors, ensuring the efficient turnover of ribosomes .
Studies have shown that depletion of mtRRF in human cell lines leads to severe mitochondrial dysfunction, including mitochondrial dysmorphism, aggregation of mitoribosomes, elevated mitochondrial superoxide production, and loss of oxidative phosphorylation complexes . These findings highlight the essential role of mtRRF in maintaining mitochondrial health and cellular viability.
Recombinant human mtRRF is used in research to study mitochondrial translation and its regulation. It provides insights into the mechanisms of mitochondrial diseases and potential therapeutic targets for conditions associated with mitochondrial dysfunction .
