MRPL48 Human

Mitochondrial Ribosomal Protein L48 (MRPL48) is a crucial component of the mitochondrial ribosome, specifically the large 39S subunit. Mitochondrial ribosomes, or mitoribosomes, are essential for protein synthesis within the mitochondria, the powerhouse of the cell. Unlike prokaryotic ribosomes, mitoribosomes have a higher protein-to-rRNA ratio and lack a 5S rRNA component .

MRPL48 is encoded by nuclear genes and is imported into the mitochondria where it integrates into the mitoribosome. The protein plays a significant role in the translation of mitochondrial mRNA, which is vital for the production of proteins involved in the mitochondrial respiratory chain .

Recombinant MRPL48 is typically produced using bacterial expression systems such as Escherichia coli (E. coli). The gene encoding MRPL48 is cloned into an expression vector, which is then introduced into E. coli cells. The bacteria are cultured, and the protein is expressed and subsequently purified using affinity chromatography techniques. The recombinant protein often includes a His-tag to facilitate purification .

The recombinant MRPL48 protein is analyzed using various biochemical techniques to confirm its purity and functionality. Common methods include:

• SDS-PAGE: This technique is used to determine the purity and molecular weight of the protein.
• Western Blotting: This method is employed to verify the presence of the His-tag and to confirm the identity of the protein.
• Mass Spectrometry: This technique provides detailed information about the protein’s molecular weight and post-translational modifications .

Recombinant MRPL48 is used in various research applications, including studies on mitochondrial function, protein synthesis, and the role of mitoribosomes in health and disease. It serves as a valuable tool for understanding the molecular mechanisms underlying mitochondrial disorders and for developing potential therapeutic strategies .

Recombinant MRPL48 protein is typically stored in a buffer solution containing Tris-HCl and glycerol to maintain its stability. It should be stored at -20°C or -70°C for long-term storage and should avoid repeated freeze-thaw cycles to prevent degradation .