MRPL28 Human

Mitochondrial Ribosomal Protein L28 Human Recombinant
Cat. No.
BT13545
Source
E.coli.
Synonyms
MAAT1, p15, 39S ribosomal protein L28, mitochondrial, L28mt, MRP-L28, Melanoma-associated antigen recognized by T-lymphocytes.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MRPL28 Human Recombinant produced in E. coli is a single polypeptide chain containing 222 amino acids (56-256) and having a molecular mass of 25.8 kDa. MRPL28 is fused to 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
MRPL28, a member of the ribosomal protein L28P family, is present in various tissues like spleen, testis, thymus, liver, kidney, brain, adrenal, lung, and retinal tissue. This mitochondrial 39S ribosomal protein is encoded by nuclear genes and participates in mitochondrial protein synthesis. Mitoribosomes, composed of a small 28S and a large 39S subunit, rely on MRPL28 for their function. MRPL28 holds therapeutic significance for HLA-A24 (A24) patients as it is recognized by the tumor-infiltrating lymphocyte (TIL) 1290, known to target the A24 serotype.
Description
Recombinant human MRPL28, produced in E. coli, is a single polypeptide chain with a molecular weight of 25.8 kDa. It consists of 222 amino acids (56-256) and has a 21 amino acid His-tag fused at its N-terminus. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The MRPL28 solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.5), 0.1M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined by SDS-PAGE analysis and exceeds 95%.
Synonyms
MAAT1, p15, 39S ribosomal protein L28, mitochondrial, L28mt, MRP-L28, Melanoma-associated antigen recognized by T-lymphocytes.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MNGQRERVED VPIPIYFPPE SQRGLWGGEG WILGQIYANN DKLSKRLKKV WKPQLFEREF YSEILDKKFT VTVTMRTLDL IDEAYGLDFY ILKTPKEDLC SKFGMDLKRG MLLRLARQDP QLHPEDPERR AAIYDKYKEF AIPEEEAEWV GLTLEEAIEK QRLLEEKDPV PLFKIYVAEL IQQLQQQALS EPAVVQKRAS GQ.

Product Science Overview

Introduction

Mitochondrial Ribosomal Protein L28 (MRPL28) is a protein encoded by the MRPL28 gene in humans. This protein is a component of the mitochondrial ribosome, specifically the large 39S subunit . Mitochondrial ribosomes, also known as mitoribosomes, are essential for protein synthesis within the mitochondria, the powerhouse of the cell .

Structure and Function

Mitoribosomes are distinct from their prokaryotic counterparts in several ways. They consist of a small 28S subunit and a large 39S subunit, with an estimated 75% protein to rRNA composition, compared to prokaryotic ribosomes where this ratio is reversed . Unlike prokaryotic ribosomes, mitoribosomes do not contain a 5S rRNA . The MRPL28 protein is part of the 39S subunit and plays a crucial role in the structural integrity and function of the mitoribosome .

Genetic Encoding and Expression

The MRPL28 gene is encoded by nuclear DNA, even though the protein functions within the mitochondria . This gene is involved in pathways related to mitochondrial translation and protein metabolism . The expression of MRPL28 is observed in various tissues, with low tissue specificity . It is localized predominantly in the mitochondria, contributing to the overall protein synthesis machinery within this organelle .

Clinical Significance

Mutations or dysregulation of the MRPL28 gene can be associated with certain diseases. For instance, it has been linked to conditions such as Ascaridiasis and Nail Disorder, Nonsyndromic Congenital, 4 . Understanding the function and regulation of MRPL28 is crucial for insights into mitochondrial biology and related pathologies.

Recombinant MRPL28

Recombinant MRPL28 refers to the protein produced through recombinant DNA technology, which involves inserting the MRPL28 gene into an expression system to produce the protein in vitro. This recombinant protein is used in various research applications to study its function, interactions, and role in mitochondrial protein synthesis .

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