MRPL1 Human
Mitochondrial Ribosomal Protein L1 Human Recombinant
Cat. No.
BT13352
Source
E.coli.
Synonyms
BM022, L1MT, MRP-L1, 39S ribosomal protein L1, mitochondria, MRPL1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
MRPL1 Human Recombinant produced in E. coli is a single polypeptide chain containing 298 amino acids (51-325) and having a molecular mass of 33.8kDa. MRPL1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Mitochondrial Ribosomal Protein L1 (MRPL1) is a crucial component of the large (39S) subunit of mitochondrial ribosomes, which are essential for protein synthesis within mitochondria. These ribosomes, distinct from cytoplasmic ribosomes, are composed of a 39S large subunit and a 28S small subunit. MRPL1, alongside other mitochondrial ribosomal proteins (MRPs), plays a vital role in this process. The MRPL1 gene undergoes alternative splicing, resulting in two isoforms. Isoform 1 is expressed ubiquitously, while isoform 2 shows preferential expression in the heart.
Description
Recombinant human MRPL1, expressed in E. coli, is available as a purified single polypeptide chain. This protein encompasses amino acids 51 to 325 of the MRPL1 sequence, resulting in a molecular weight of 33.8 kDa. For purification and detection purposes, a 23-amino acid His-tag is fused to the N-terminus. The protein is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The MRPL1 solution is provided at a concentration of 0.5 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 30% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the MRPL1 protein is greater than 80%, as assessed by SDS-PAGE analysis.
Synonyms
BM022, L1MT, MRP-L1, 39S ribosomal protein L1, mitochondria, MRPL1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSKKTKKGA KEKTPDEKKD EIEKIKAYPY MEGEPEDDVY LKRLYPRQIY EVEKAVHLLK KFQILDFTSP KQSVYLDLTL DMALGKKKNV EPFTSVLSLP YPFASEINKV AVFTENASEV KIAEENGAAF AGGTSLIQKI WDDEIVADFY VAVPEIMPEL NRLRKKLNKK YPKLSRNSIG RDIPKMLELF KNGHEIKVDE ERENFLQTKI ATLDMSSDQI AANLQAVINE VCRHRPLNLG PFVVRAFLRS STSEGLLLKI DPLLPKEVKN EESEKEDA.
Product Science Overview
Introduction
Gene and Protein Structure
The MRPL1 gene is located on chromosome 4q21.1 in humans . It encodes a protein that is part of the L1 ribosomal protein family. The mitochondrial ribosome, or mitoribosome, consists of a small 28S subunit and a large 39S subunit. Unlike prokaryotic ribosomes, mitoribosomes have a higher protein to rRNA ratio (approximately 75% protein) and lack a 5S rRNA .
Function
Pathways
Clinical Significance
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
