MRPL13 Human

Mitochondrial Ribosomal Protein L13 Human Recombinant
Cat. No.
BT13417
Source
E.coli.
Synonyms
L13, L13A, L13mt, RPL13, RPML13, 39S ribosomal protein L13, mitochondrial, MRPL13.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MRPL13 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-178 a.a.) and having a molecular mass of 23.1kDa. MRPL13 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Mitochondrial ribosomal proteins, found in mammals, are produced by genes located in the cell nucleus. These proteins play a crucial role in protein synthesis within the mitochondria. Mammalian mitochondrial ribosomes, known as mitoribosomes, consist of a smaller 28S subunit and a larger 39S subunit. MRPL13 is a protein component of the 39S subunit. In contrast to prokaryotic ribosomes, where rRNA makes up the majority, mitoribosomes have a higher protein-to-rRNA ratio, estimated at 75%. Another distinction is the presence of 5S rRNA in prokaryotic ribosomes, which is absent in mammalian mitoribosomes. The proteins that make up mitoribosomes vary significantly in their amino acid sequence and occasionally in their biochemical properties across different species. This difference makes it difficult to identify them solely based on sequence similarity.
Description
Recombinant MRPL13, of human origin, is produced in E. coli. It is a single polypeptide chain that lacks glycosylation. This protein consists of 201 amino acids (with amino acids 1-178 representing the MRPL13 sequence) and has a molecular weight of 23.1 kDa. A 23-amino acid His-tag is fused to the N-terminus of MRPL13 to facilitate purification, which is achieved using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The MRPL13 protein solution has a concentration of 1 mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.4M urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the protein can be stored at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. It is important to avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
L13, L13A, L13mt, RPL13, RPML13, 39S ribosomal protein L13, mitochondrial, MRPL13.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSFSRA PQQWATFARI WYLLDGKMQP PGKLAAMASI RLQGLHKPVY HALSDCGDHV VIMNTRHIAF SGNKWEQKVY SSHTGYPGGF RQVTAAQLHL RDPVAIVKLA IYGMLPKNLH RRTMMERLHL FPDEYIPEDI LKNLVEELPQ PRKIPKRLDE YTQEEIDAFP RLWTPPEDYR L.

Product Science Overview

Introduction

Mitochondrial Ribosomal Protein L13 (MRPL13) is a protein encoded by the MRPL13 gene in humans. This protein is a component of the mitochondrial ribosome, specifically the large 39S subunit . Mitochondrial ribosomes, also known as mitoribosomes, are responsible for protein synthesis within the mitochondria, which are the powerhouses of the cell .

Function

Mammalian mitochondrial ribosomal proteins, including MRPL13, are encoded by nuclear genes and play a crucial role in the synthesis of proteins within the mitochondrion . Mitoribosomes consist of a small 28S subunit and a large 39S subunit, with an estimated 75% protein to rRNA composition . This ratio is reversed in prokaryotic ribosomes, which also contain a 5S rRNA . The proteins comprising the mitoribosome differ greatly in sequence and biochemical properties among different species, making recognition by sequence homology challenging .

Gene Location and Expression

The MRPL13 gene is located on chromosome 8 in humans . It is expressed in various tissues, including the ganglionic eminence, right adrenal gland, islet of Langerhans, rectum, stromal cell of the endometrium, left adrenal gland, Achilles tendon, left ventricle, right ventricle, and right lobe of the liver .

Biological Processes

MRPL13 is involved in several biological processes, including mitochondrial translational elongation, mitochondrial translational termination, protein biosynthesis, and the negative regulation of translation . It is a structural constituent of the ribosome and is involved in RNA binding .

Clinical Significance

Diseases associated with MRPL13 include animal phobia and mucolipidosis II alpha/beta . The gene is also involved in pathways related to mitochondrial translation and metabolism of proteins .

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