MRM1 Human
Mitochondrial RRNA Methyltransferase 1 Human Recombinant
Cat. No.
BT25590
Source
E.coli.
Synonyms
Mitochondrial rRNA methyltransferase 1 homolog, rRNA methyltransferase 1, mitochondrial, Mitochondrial large ribosomal RNA ribose methylase, MRM1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
MRM1 Human Recombinant produced in E. coli is a single polypeptide chain containing 356 amino acids (21-353) and having a molecular mass of 38.8kDa. MRM1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Mitochondrial rRNA methyltransferase 1 homolog (MRM1), a member of the RNA methyltransferase TrmH family, is responsible for methylating the ribose of guanosine G-2270 within the peptidyl transferase center of the mitochondrial large ribosomal RNA (21S).
Description
Recombinant MRM1 protein, produced in E. coli, is a single polypeptide chain with a molecular weight of 38.8 kDa. This protein consists of 356 amino acids (residues 21-353) and includes a 23 amino acid His-tag fused at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The MRM1 solution is provided at a concentration of 0.25 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 30% glycerol, 1 mM DTT, and 0.1 mM PMSF.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. It is important to avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90% as determined by SDS-PAGE analysis.
Synonyms
Mitochondrial rRNA methyltransferase 1 homolog, rRNA methyltransferase 1, mitochondrial, Mitochondrial large ribosomal RNA ribose methylase, MRM1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSSHAARHG ERPGGEELSR LLLDDLVPTS RLELLFGMTP CLLALQAARR SVARLLLQAG KAGLQGKRAE LLRMAEARDI PVLRPRRQKL DTMCRYQVHQ GVCMEVSPLR PRPWREAGEA SPGDDPQQLW LVLDGIQDPR NFGAVLRSAH FLGVDKVITS RRNSCPLTPV VSKSSAGAME VMDVFSTDDL TGFLQTKAQQ GWLVAGTVGC PSTEDPQSSE IPIMSCLEFL WERPTLLVLG NEGSGLSQEV QASCQLLLTI LPRRQLPPGL ESLNVSVAAG ILLHSICSQR KGFPTEGERR QLLQDPQEPS ARSEGLSMAQ HPGLSSGPEK ERQNEG.
Product Science Overview
Gene and Protein Information
- Gene Symbol: MRM1
- Aliases: 16S RRNA (Guanosine (1145)-2’-O)-Methyltransferase, RRNA Methyltransferase 1, Mitochondrial, FLJ22578, Mitochondrial Large Ribosomal RNA Ribose Methylase .
- Enzyme Classification: EC 2.1.1.- .
- Protein Structure: The recombinant human MRM1 protein is often expressed with an N-terminal His-tag for purification purposes. It consists of amino acids 21-353 and has a theoretical molecular weight of approximately 38.8 kDa .
Function and Mechanism
MRM1 is an S-adenosyl-L-methionine-dependent 2’-O-ribose methyltransferase. It catalyzes the formation of 2’-O-methylguanosine at position 1145 in the 16S mtLSU rRNA . This methylation is essential for the proper functioning of the mitochondrial ribosome, particularly in the peptidyl transferase center, which is crucial for protein synthesis within mitochondria .
Biological Significance
Associated Diseases
Research and Applications
Recombinant human MRM1 protein is widely used in research to study its function and role in mitochondrial biology. It is typically expressed in E. coli and purified using conventional chromatography techniques . This recombinant protein is valuable for investigating the mechanisms of rRNA methylation and its impact on mitochondrial function.
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